Short Communication: Specimen Processing Impacts Tissue Tenofovir Pharmacokinetic Measurements

Rahul P. Bakshi, Jennifer Breakey, Madhuri Manohar, Bhavna Jois, Edward J. Fuchs, Mark A. Marzinke

Research output: Contribution to journalArticlepeer-review

Abstract

Antiretroviral drug concentrations at sites of HIV exposure are important drivers that influence the development of HIV pre-exposure chemoprophylaxis strategies and regimens. We assessed the effect of collection method - in the presence or absence of tissue culture medium - on tenofovir (TFV) and tenofovir diphosphate (TFV-DP) concentrations in colonic biopsies. We find significant baseline interbiopsy variation in TFV (38% CV) and TFV-DP (33% CV) concentrations. Incubation in medium leads to a fluid absorption-driven twofold increase in tissue weight with a concomitant 75% decrease in weight-adjusted tissue TFV concentrations 120 min post-incubation. In contrast, adjusted TFV-DP concentrations decrease by only 25% during the same period, with this difference not achieving statistical significance. Although colonic biopsies should be collected in the absence of medium for accurate TFV concentrations, the presence of medium does not significantly impact TFV-DP-dependent pharmacokinetic or pharmacodynamic assays. Appropriate assessment of tissue drug concentrations should account for biopsy collection method and drug mechanism of action.

Original languageEnglish (US)
Pages (from-to)354-356
Number of pages3
JournalAIDS research and human retroviruses
Volume34
Issue number4
DOIs
StatePublished - Apr 2018

Keywords

  • PK/PD
  • antiretroviral
  • biopsy
  • tenofovir
  • tissue

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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