The interaction of aflatoxin B2 (AFB2) in vivo with rat liver nuclear macromolecules was examined in an attempt to correlate this binding with biological potency. The incorporation of [3H]AFB2 residues into rat liver his tones and DNA was determined 2, 24 and 48 h following administration of a single i.p. dose of 1 mg (3H)AFB2/kg body weight. At each time point, hist one H1 and the total histone fraction contained 5-30-fold more [3H]AFB2 moieties than did DNA on a weight basis. Analytical reversed-phase h.p.l.c. of the acid hydrolysis products resulting from AFB2 binding to DNA revealed that 85% of the radioactivity co-chromatographed with the major aflatoxin B1-DNA adduct, 2,3-dinydro-2-(N7-guanyl)-3-hydroxyaftatoxin B1. These studies revealed an apparent correlation between AFB2 derived binding to DNA in vivo in rats and its potency as a toxin and carcinogen in this species.
ASJC Scopus subject areas
- Cancer Research