Short-chain fatty acid activation by acyl-coenzyme A synthetases requires SIR2 protein function in Salmonella enterica and Saccharomyces cerevisiae

Vincent J. Starai, Hidekazu Takahashi, Jef D. Boeke, Jorge C. Escalante-Semerena

Research output: Contribution to journalArticle

Abstract

SIR2 proteins have NAD+-dependent histone deacetylase activity, but no metabolic role has been assigned to any of these proteins. In Salmonella enterica, SIR2 function was required for activity of the acetyl-CoA synthetase (Acs) enzyme. A greater than two orders of magnitude increase in the specific activity of Acs enzyme synthesized by a sirtuin-deficient strain was measured after treatment with homogeneous S. enterica SIR2 protein. Human SIR2A and yeast SIR2 proteins restored growth of SIR2-deficient S. enterica on acetate and propionate, suggesting that eukaryotic cells may also use SIR2 proteins to control the synthesis of acetyl-CoA by the level of acetylation of acetyl-CoA synthetases. Consistent with this idea, growth of a quintuple sir2 hst1 hst2 hst3 hst4 mutant strain of the yeast Saccharomyces cerevisiae on acetate or propionate was severely impaired. The data suggest that the Hst3 and Hst4 proteins are the most important for allowing growth on these short-chain fatty acids.

Original languageEnglish (US)
Pages (from-to)545-555
Number of pages11
JournalGenetics
Volume163
Issue number2
StatePublished - Feb 1 2003

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Coenzyme A Ligases
Salmonella enterica
Volatile Fatty Acids
Acetate-CoA Ligase
Saccharomyces cerevisiae
Propionates
Proteins
Acetates
Growth
Acetyl Coenzyme A
Histone Deacetylases
Fungal Proteins
Eukaryotic Cells
Enzymes
Acetylation
NAD
Yeasts

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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Short-chain fatty acid activation by acyl-coenzyme A synthetases requires SIR2 protein function in Salmonella enterica and Saccharomyces cerevisiae. / Starai, Vincent J.; Takahashi, Hidekazu; Boeke, Jef D.; Escalante-Semerena, Jorge C.

In: Genetics, Vol. 163, No. 2, 01.02.2003, p. 545-555.

Research output: Contribution to journalArticle

Starai, Vincent J. ; Takahashi, Hidekazu ; Boeke, Jef D. ; Escalante-Semerena, Jorge C. / Short-chain fatty acid activation by acyl-coenzyme A synthetases requires SIR2 protein function in Salmonella enterica and Saccharomyces cerevisiae. In: Genetics. 2003 ; Vol. 163, No. 2. pp. 545-555.
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