Shigella dysenteriae Type 1-Specific Bacteriophage from Environmental Waters in Bangladesh

Shah M. Faruque, Nityananda Chowdhury, Rasel Khan, M. Rubayet Hasan, Jebun Nahar, M. Johirul Islam, Shinji Yamasaki, A. N. Ghosh, G. Balakrish Nair, David A. Sack

Research output: Contribution to journalArticle

Abstract

Shigella dysenteriae type 1 is the causative agent of the most severe form of bacillary dysentery, which occurs as epidemics in many developing countries. We isolated a bacteriophage from surface water samples from Bangladesh that specifically lyses strains of S. dysenteriae type 1. This phage, designated SF-9, belongs to the Podoviridae family and has a 41-kb double-stranded DNA genome. Further screening of water samples for the prevalence of the phage revealed 9 of 71 (12.6%) water samples which were positive for the phage. These water samples were also positive in PCR assays for one or more S. dysenteriae type 1-specific genes, including ipaBCD and stx1, and live S. dysenteriae type 1 was isolated from three phage-positive samples. The results of this study suggest that phage SF-9 may have epidemiological applications in tracing the presence of S. dysenteriae type 1 in environmental waters.

Original languageEnglish (US)
Pages (from-to)7028-7031
Number of pages4
JournalApplied and Environmental Microbiology
Volume69
Issue number12
DOIs
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Shigella dysenteriae
Bangladesh
bacteriophage
bacteriophages
Bacteriophages
Water
dysentery
water
Podoviridae
lysis
sampling
Bacillary Dysentery
genome
developing world
assay
surface water
DNA
Developing Countries
developing countries
gene

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Shigella dysenteriae Type 1-Specific Bacteriophage from Environmental Waters in Bangladesh. / Faruque, Shah M.; Chowdhury, Nityananda; Khan, Rasel; Hasan, M. Rubayet; Nahar, Jebun; Islam, M. Johirul; Yamasaki, Shinji; Ghosh, A. N.; Nair, G. Balakrish; Sack, David A.

In: Applied and Environmental Microbiology, Vol. 69, No. 12, 12.2003, p. 7028-7031.

Research output: Contribution to journalArticle

Faruque, SM, Chowdhury, N, Khan, R, Hasan, MR, Nahar, J, Islam, MJ, Yamasaki, S, Ghosh, AN, Nair, GB & Sack, DA 2003, 'Shigella dysenteriae Type 1-Specific Bacteriophage from Environmental Waters in Bangladesh', Applied and Environmental Microbiology, vol. 69, no. 12, pp. 7028-7031. https://doi.org/10.1128/AEM.69.12.7028-7031.2003
Faruque, Shah M. ; Chowdhury, Nityananda ; Khan, Rasel ; Hasan, M. Rubayet ; Nahar, Jebun ; Islam, M. Johirul ; Yamasaki, Shinji ; Ghosh, A. N. ; Nair, G. Balakrish ; Sack, David A. / Shigella dysenteriae Type 1-Specific Bacteriophage from Environmental Waters in Bangladesh. In: Applied and Environmental Microbiology. 2003 ; Vol. 69, No. 12. pp. 7028-7031.
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