Shiga toxin-producing Escherichia coli isolated from patients with diarrhoea in Bangladesh

M. Aminul Islam, A. E. Heuvelink, E. De Boer, P. D. Sturm, R. R. Beumer, M. H. Zwietering, A. S G Faruque, R. Haque, David Allen Sack, K. A. Talukder

Research output: Contribution to journalArticle

Abstract

The prevalence of Shiga toxin-producing Escherichia coli (STEC) and its characteristics were determined among hospitalized patients with diarrhoea and children with diarrhoea in an urban slum community of Dhaka city using sensitive culture and PCR methods. Stool samples were collected from 410 patients with diarrhoea enrolled in the 2% surveillance system (every 50th patient attending the hospital with diarrhoeal disease is included) at the ICDDR,B hospital and from 160 children of 2-5 years of age with diarrhoea living in an urban slum in Dhaka, between September 2004 and April 2005. Shiga toxin genes (stx) were detected by multiplex PCR in the enrichment broth of nine samples (2.2%) from hospitalized patients and 11 samples (6.9%) from the community patients. STEC was isolated from five stool samples with positive PCR results using a colony patch technique. All five isolates were positive in the Vero cell assay and PCR fragments of stx genes were confirmed by sequencing. Two isolates were positive for the E. coli attaching-and-effacing (eae) gene and four were positive for the enterohaemolysin (hlyEHEC) gene and enterohaemolysin production. The five isolates belonged to five different serotypes: O32:H25, O2:H45, O76:H19, ONT:H25 and ONT:H19. It can be concluded that STEC is not a common pathogen in Bangladesh among hospitalized patients with diarrhoea nor among mild cases of diarrhoea in the community.

Original languageEnglish (US)
Pages (from-to)380-385
Number of pages6
JournalJournal of Medical Microbiology
Volume56
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Shiga-Toxigenic Escherichia coli
Bangladesh
Diarrhea
Shiga Toxin
Poverty Areas
Genes
Polymerase Chain Reaction
Vero Cells
Multiplex Polymerase Chain Reaction
Escherichia coli

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Aminul Islam, M., Heuvelink, A. E., De Boer, E., Sturm, P. D., Beumer, R. R., Zwietering, M. H., ... Talukder, K. A. (2007). Shiga toxin-producing Escherichia coli isolated from patients with diarrhoea in Bangladesh. Journal of Medical Microbiology, 56(3), 380-385. https://doi.org/10.1099/jmm.0.46916-0

Shiga toxin-producing Escherichia coli isolated from patients with diarrhoea in Bangladesh. / Aminul Islam, M.; Heuvelink, A. E.; De Boer, E.; Sturm, P. D.; Beumer, R. R.; Zwietering, M. H.; Faruque, A. S G; Haque, R.; Sack, David Allen; Talukder, K. A.

In: Journal of Medical Microbiology, Vol. 56, No. 3, 03.2007, p. 380-385.

Research output: Contribution to journalArticle

Aminul Islam, M, Heuvelink, AE, De Boer, E, Sturm, PD, Beumer, RR, Zwietering, MH, Faruque, ASG, Haque, R, Sack, DA & Talukder, KA 2007, 'Shiga toxin-producing Escherichia coli isolated from patients with diarrhoea in Bangladesh', Journal of Medical Microbiology, vol. 56, no. 3, pp. 380-385. https://doi.org/10.1099/jmm.0.46916-0
Aminul Islam M, Heuvelink AE, De Boer E, Sturm PD, Beumer RR, Zwietering MH et al. Shiga toxin-producing Escherichia coli isolated from patients with diarrhoea in Bangladesh. Journal of Medical Microbiology. 2007 Mar;56(3):380-385. https://doi.org/10.1099/jmm.0.46916-0
Aminul Islam, M. ; Heuvelink, A. E. ; De Boer, E. ; Sturm, P. D. ; Beumer, R. R. ; Zwietering, M. H. ; Faruque, A. S G ; Haque, R. ; Sack, David Allen ; Talukder, K. A. / Shiga toxin-producing Escherichia coli isolated from patients with diarrhoea in Bangladesh. In: Journal of Medical Microbiology. 2007 ; Vol. 56, No. 3. pp. 380-385.
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abstract = "The prevalence of Shiga toxin-producing Escherichia coli (STEC) and its characteristics were determined among hospitalized patients with diarrhoea and children with diarrhoea in an urban slum community of Dhaka city using sensitive culture and PCR methods. Stool samples were collected from 410 patients with diarrhoea enrolled in the 2{\%} surveillance system (every 50th patient attending the hospital with diarrhoeal disease is included) at the ICDDR,B hospital and from 160 children of 2-5 years of age with diarrhoea living in an urban slum in Dhaka, between September 2004 and April 2005. Shiga toxin genes (stx) were detected by multiplex PCR in the enrichment broth of nine samples (2.2{\%}) from hospitalized patients and 11 samples (6.9{\%}) from the community patients. STEC was isolated from five stool samples with positive PCR results using a colony patch technique. All five isolates were positive in the Vero cell assay and PCR fragments of stx genes were confirmed by sequencing. Two isolates were positive for the E. coli attaching-and-effacing (eae) gene and four were positive for the enterohaemolysin (hlyEHEC) gene and enterohaemolysin production. The five isolates belonged to five different serotypes: O32:H25, O2:H45, O76:H19, ONT:H25 and ONT:H19. It can be concluded that STEC is not a common pathogen in Bangladesh among hospitalized patients with diarrhoea nor among mild cases of diarrhoea in the community.",
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