Shiga toxin is transported into the nucleoli of intestinal epithelial cells via a carrier-dependent process

Boris Baibakov, Rakhilya Murtazina, Christian Elowsky, Francis M. Giardiello, Olga Kovbasnjuk

Research output: Contribution to journalArticlepeer-review

Abstract

Shiga toxin (Stx) produced by the invasive Shigella dysenteriae serotype 1 (S. dysenteriae1) causes gastrointestinal and kidney complications. It has been assumed that Stx is released intracellularly after enterocyte invasion by S. dysenteriae1. However, there is little information about Stx distribution inside S. dysenteriae1-infected enterocytes. Here, we use intestinal epithelial T84 cells to characterize the trafficking of Stx delivered into the cytosol, in ways that mimic aspects of S. dysenteriae1 infection. We find that cytoplasmic Stx is transported into nucleoli. Stx nucleolar movement is carrier- and energy-dependent. Stx binding to the nucleoli of normal human enterocytes in vitro supports possible roles for nucleolar trafficking in toxin-induced intestinal pathology. 2010 by the authors; licensee MDPI, Basel, Switzerland.

Original languageEnglish (US)
Pages (from-to)1318-1335
Number of pages18
JournalToxins
Volume2
Issue number6
DOIs
StatePublished - Jun 2010

Keywords

  • Intestinal epithelial cells
  • Nucleolar trafficking
  • Shiga toxin

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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