Shear stress regulates aquaporin-5 and airway epithelial barrier function

Research output: Contribution to journalArticlepeer-review

Abstract

As the interface with the outside world, the airway epithelial barrier is critical to lung defense. Because of respiratory efforts, the airways are exposed to shear stress; however, little is known regarding the effects of shear on epithelial function. We report that low-level shear stress enhances epithelial barrier function, an effect that requires serial activation of the transient receptor potential vanilloid (TRPV) 4 and L-type voltage-gated calcium channel (VGCC) and an increase in intracellular calcium. These changes lead to a selective decrease in aquaporin-5 (AQP5) abundance because of protein internalization and degradation. To determine whether AQP5 plays a role in mediating the shear effects on paracellular permeability, we overexpressed hAQP5 in 16HBE cells, an airway epithelial cell line without endogenous AQP5. We found that AQP5 expression was needed for shear-induced barrier enhancement. These findings have direct relevance to the regulation of epithelial barrier function, membrane permeability, and water homeostasis in the respiratory epithelia.

Original languageEnglish (US)
Pages (from-to)3345-3350
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number9
DOIs
StatePublished - Mar 4 2008

Keywords

  • Cytoskeleton
  • Epithelium
  • Permeability
  • Transient receptor potential vanilloid
  • Voltage-gated calcium channel

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Shear stress regulates aquaporin-5 and airway epithelial barrier function'. Together they form a unique fingerprint.

Cite this