Sex differences in projections from preoptic area aromatase cells to the periaqueductal gray in Japanese quail

Claudio Carere, Gregory F. Ball, Jacques Balthazart

Research output: Contribution to journalArticle

Abstract

In many vertebrate species the medial preoptic area projects to a premotor nucleus, the periaqueductal central gray (PAG). This connection plays an important role in the control of reproductive behavior. In male Japanese quail (Coturnix japonica) specifically, the medial preoptic nucleus (POM), where various types of sensory inputs converge, is a critical site for the activational action of testosterone on male sexual behavior. To activate male copulatory behavior, testosterone must be aromatized to estradiol within the POM and aromatase-immunoreactive cells in the POM are the main source of projections to the PAG. The POM-PAG connection is thus an important functional circuit integrating the sensory with premotor components of sexual behavior. Contrary to what is observed in males, testosterone does not activate male-typical copulatory behavior in females and we investigated here via retrograde tracing methods whether this behavioral sexual difference is associated with a sex difference in connectivity between POM and PAG. Fluorescent microspheres were injected in the PAG of male and female quail and retrogradely labeled fluorescent cells counted in four fields of the POM in sections that had been immunolabeled for aromatase. Males had more aromatase-immunoreactive neurons projecting to the PAG than females and this difference was most prominent in the caudolateral part of the nucleus that has been specifically implicated in the control of male copulatory behavior. These data therefore support the hypothesis that sex differences in POM-PAG connectivity are causally linked to the sex difference in the behavioral response to testosterone.

Original languageEnglish (US)
Pages (from-to)894-907
Number of pages14
JournalJournal of Comparative Neurology
Volume500
Issue number5
DOIs
StatePublished - Feb 10 2007

Fingerprint

Coturnix
Periaqueductal Gray
Preoptic Area
Aromatase
Sex Characteristics
Testosterone
Sexual Behavior
Reproductive Behavior
Quail
Microspheres
Vertebrates
Estradiol
Neurons

Keywords

  • Medial preoptic nucleus
  • Mesencephalic central gray
  • Retrograde tracing
  • Sexual behavior
  • Sexual differentiation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sex differences in projections from preoptic area aromatase cells to the periaqueductal gray in Japanese quail. / Carere, Claudio; Ball, Gregory F.; Balthazart, Jacques.

In: Journal of Comparative Neurology, Vol. 500, No. 5, 10.02.2007, p. 894-907.

Research output: Contribution to journalArticle

Carere, Claudio ; Ball, Gregory F. ; Balthazart, Jacques. / Sex differences in projections from preoptic area aromatase cells to the periaqueductal gray in Japanese quail. In: Journal of Comparative Neurology. 2007 ; Vol. 500, No. 5. pp. 894-907.
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