Sex differences in gene regulation in the dorsal root ganglion after nerve injury 11 Medical and Health Sciences 1109 Neurosciences 06 Biological Sciences 0604 Genetics

Kimberly E. Stephens, Weiqiang Zhou, Zhicheng Ji, Zhiyong Chen, Shao-Qiu He, Hong Kai Ji, Yun Guan, Sean Dixon Taverna

Research output: Contribution to journalArticle

Abstract

Background: Pain is a subjective experience derived from complex interactions among biological, environmental, and psychosocial pathways. Sex differences in pain sensitivity and chronic pain prevalence are well established. However, the molecular basis underlying these sex dimorphisms are poorly understood particularly with regard to the role of the peripheral nervous system. Here we sought to identify shared and distinct gene networks functioning in the peripheral nervous systems that may contribute to sex differences of pain in rats after nerve injury. Results: We performed RNA-seq on dorsal root ganglia following chronic constriction injury of the sciatic nerve in male and female rats. Analysis from paired naive and injured tissues showed that 1513 genes were differentially expressed between sexes. Genes which facilitated synaptic transmission in naïve and injured females did not show increased expression in males. Conclusions: Appreciating sex-related gene expression differences and similarities in neuropathic pain models may help to improve the translational relevance to clinical populations and efficacy of clinical trials of this major health issue.

Original languageEnglish (US)
Article number147
JournalBMC genomics
Volume20
Issue number1
DOIs
StatePublished - Feb 19 2019

Fingerprint

Biological Science Disciplines
Spinal Ganglia
Neurosciences
Sex Characteristics
Peripheral Nervous System
Pain
Health
Wounds and Injuries
Genes
Gene Regulatory Networks
Neuralgia
Sciatic Nerve
Constriction
Synaptic Transmission
Chronic Pain
Clinical Trials
RNA
Gene Expression
Population

Keywords

  • Dorsal root ganglion
  • Gene expression
  • Nerve injury
  • Peripheral nervous system
  • RNA-seq
  • Sex differences

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

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title = "Sex differences in gene regulation in the dorsal root ganglion after nerve injury 11 Medical and Health Sciences 1109 Neurosciences 06 Biological Sciences 0604 Genetics",
abstract = "Background: Pain is a subjective experience derived from complex interactions among biological, environmental, and psychosocial pathways. Sex differences in pain sensitivity and chronic pain prevalence are well established. However, the molecular basis underlying these sex dimorphisms are poorly understood particularly with regard to the role of the peripheral nervous system. Here we sought to identify shared and distinct gene networks functioning in the peripheral nervous systems that may contribute to sex differences of pain in rats after nerve injury. Results: We performed RNA-seq on dorsal root ganglia following chronic constriction injury of the sciatic nerve in male and female rats. Analysis from paired naive and injured tissues showed that 1513 genes were differentially expressed between sexes. Genes which facilitated synaptic transmission in na{\"i}ve and injured females did not show increased expression in males. Conclusions: Appreciating sex-related gene expression differences and similarities in neuropathic pain models may help to improve the translational relevance to clinical populations and efficacy of clinical trials of this major health issue.",
keywords = "Dorsal root ganglion, Gene expression, Nerve injury, Peripheral nervous system, RNA-seq, Sex differences",
author = "Stephens, {Kimberly E.} and Weiqiang Zhou and Zhicheng Ji and Zhiyong Chen and Shao-Qiu He and Ji, {Hong Kai} and Yun Guan and Taverna, {Sean Dixon}",
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AU - Stephens, Kimberly E.

AU - Zhou, Weiqiang

AU - Ji, Zhicheng

AU - Chen, Zhiyong

AU - He, Shao-Qiu

AU - Ji, Hong Kai

AU - Guan, Yun

AU - Taverna, Sean Dixon

PY - 2019/2/19

Y1 - 2019/2/19

N2 - Background: Pain is a subjective experience derived from complex interactions among biological, environmental, and psychosocial pathways. Sex differences in pain sensitivity and chronic pain prevalence are well established. However, the molecular basis underlying these sex dimorphisms are poorly understood particularly with regard to the role of the peripheral nervous system. Here we sought to identify shared and distinct gene networks functioning in the peripheral nervous systems that may contribute to sex differences of pain in rats after nerve injury. Results: We performed RNA-seq on dorsal root ganglia following chronic constriction injury of the sciatic nerve in male and female rats. Analysis from paired naive and injured tissues showed that 1513 genes were differentially expressed between sexes. Genes which facilitated synaptic transmission in naïve and injured females did not show increased expression in males. Conclusions: Appreciating sex-related gene expression differences and similarities in neuropathic pain models may help to improve the translational relevance to clinical populations and efficacy of clinical trials of this major health issue.

AB - Background: Pain is a subjective experience derived from complex interactions among biological, environmental, and psychosocial pathways. Sex differences in pain sensitivity and chronic pain prevalence are well established. However, the molecular basis underlying these sex dimorphisms are poorly understood particularly with regard to the role of the peripheral nervous system. Here we sought to identify shared and distinct gene networks functioning in the peripheral nervous systems that may contribute to sex differences of pain in rats after nerve injury. Results: We performed RNA-seq on dorsal root ganglia following chronic constriction injury of the sciatic nerve in male and female rats. Analysis from paired naive and injured tissues showed that 1513 genes were differentially expressed between sexes. Genes which facilitated synaptic transmission in naïve and injured females did not show increased expression in males. Conclusions: Appreciating sex-related gene expression differences and similarities in neuropathic pain models may help to improve the translational relevance to clinical populations and efficacy of clinical trials of this major health issue.

KW - Dorsal root ganglion

KW - Gene expression

KW - Nerve injury

KW - Peripheral nervous system

KW - RNA-seq

KW - Sex differences

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