Severity of post-ERCP pancreatitis directly proportional to the invasiveness of endoscopic intervention: A pilot study in a canine model

J. M. Buscaglia, B. W. Simons, B. J. Prosser, D. S. Ruben, S. A. Giday, P. Magno, J. O. Clarke, Eun Shin, Anthony N Kalloo, S. V. Kantsevoy, Kathleen L Gabrielson, S. B. Jagannath

Research output: Contribution to journalArticle

Abstract

Background and study aims: Pancreatitis complicates 1%-22% of endoscopic retrograde cholangiopancreatography procedures. The study aims were to develop a reproducible animal model of post-ERCP pancreatitis (PEP), and investigate the impact of endoscopic technique on severity of PEP. Patients and methods: ERCP was carried out in six male hound dogs. Pancreatitis was induced by one of three escalating methods: 1) pancreatic acinarization with 20-30 mL of contrast; 2) acinarization + ductal balloon occlusion + sphincterotomy; 3) acinarization + intraductal synthetic bile injection + ductal balloon occlusion + sphincterotomy. Dogs 5 and 6 received a pancreatic stent. Necropsy was performed on postoperative day 5. All pancreatic specimens were graded by two blinded pathologists according to a validated scoring system. All dogs were compared with three control dogs. Results: Dogs 1-4 developed clinical pancreatitis and hyperamylasemia (11736 vs. 722 U/L, P=0.02). Total injury scores were significantly elevated compared with controls (6.85 vs. 1.06, P=0.004). There was significant increase in acinar cell necrosis (0.86 vs. 0.06, P=

Original languageEnglish (US)
Pages (from-to)506-512
Number of pages7
JournalEndoscopy
Volume40
Issue number6
DOIs
StatePublished - Jun 2008

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Endoscopic Retrograde Cholangiopancreatography
Pancreatitis
Canidae
Dogs
Balloon Occlusion
Hyperamylasemia
Acinar Cells
Bile
Stents
Necrosis
Animal Models
Injections
Wounds and Injuries

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

Buscaglia, J. M., Simons, B. W., Prosser, B. J., Ruben, D. S., Giday, S. A., Magno, P., ... Jagannath, S. B. (2008). Severity of post-ERCP pancreatitis directly proportional to the invasiveness of endoscopic intervention: A pilot study in a canine model. Endoscopy, 40(6), 506-512. https://doi.org/10.1055/s-2007-995653

Severity of post-ERCP pancreatitis directly proportional to the invasiveness of endoscopic intervention : A pilot study in a canine model. / Buscaglia, J. M.; Simons, B. W.; Prosser, B. J.; Ruben, D. S.; Giday, S. A.; Magno, P.; Clarke, J. O.; Shin, Eun; Kalloo, Anthony N; Kantsevoy, S. V.; Gabrielson, Kathleen L; Jagannath, S. B.

In: Endoscopy, Vol. 40, No. 6, 06.2008, p. 506-512.

Research output: Contribution to journalArticle

Buscaglia, JM, Simons, BW, Prosser, BJ, Ruben, DS, Giday, SA, Magno, P, Clarke, JO, Shin, E, Kalloo, AN, Kantsevoy, SV, Gabrielson, KL & Jagannath, SB 2008, 'Severity of post-ERCP pancreatitis directly proportional to the invasiveness of endoscopic intervention: A pilot study in a canine model', Endoscopy, vol. 40, no. 6, pp. 506-512. https://doi.org/10.1055/s-2007-995653
Buscaglia, J. M. ; Simons, B. W. ; Prosser, B. J. ; Ruben, D. S. ; Giday, S. A. ; Magno, P. ; Clarke, J. O. ; Shin, Eun ; Kalloo, Anthony N ; Kantsevoy, S. V. ; Gabrielson, Kathleen L ; Jagannath, S. B. / Severity of post-ERCP pancreatitis directly proportional to the invasiveness of endoscopic intervention : A pilot study in a canine model. In: Endoscopy. 2008 ; Vol. 40, No. 6. pp. 506-512.
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