Severe acute respiratory syndrome coronavirus gene 7 products contribute to virus-induced apoptosis

Scott R. Schaecher, Erin Touchette, Jill Schriewer, R. Mark Buller, Andrew Pekosz

Research output: Contribution to journalArticle

Abstract

The proteins encoded by gene 7 of the severe acute respiratory syndrome coronavirus (SARS-CoV) have been demonstrated to have proapoptotic activity when expressed from cDNA but appear to be dispensable for virus replication. Recombinant SARS-CoVs bearing deletions in gene 7 were used to assess the contribution of gene 7 to virus replication and apoptosis in several transformed cell lines, as well as to replication and pathogenesis in golden Syrian hamsters. Deletion of gene 7 had no effect on SARS-CoV replication in transformed cell lines, nor did it alter the induction of early apoptosis markers such as annexin V binding and activation of caspase 3. However, viruses with gene 7 disruptions were not as efficient as wild-type virus in inducing DNA fragmentation, as judged by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) staining, indicating that the gene 7 products do contribute to virus-induced apoptosis. Disruption of gene 7 did not affect virus replication or morbidity in golden Syrian hamsters, suggesting that the gene 7 products are not required for acute infection in vivo. The data indicate that open reading frames 7a and 7b contribute to but are not solely responsible for the apoptosis seen in SARS-CoV-infected cells.

Original languageEnglish (US)
Pages (from-to)11054-11068
Number of pages15
JournalJournal of Virology
Volume81
Issue number20
DOIs
StatePublished - Oct 2007

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Severe Acute Respiratory Syndrome
Coronavirus
apoptosis
Apoptosis
Viruses
viruses
Virus Replication
virus replication
Transformed Cell Line
gene deletion
Genes
Mesocricetus
Gene Deletion
genes
cell lines
DNA Nucleotidylexotransferase
Annexin A5
DNA fragmentation
In Situ Nick-End Labeling
gene targeting

ASJC Scopus subject areas

  • Immunology

Cite this

Severe acute respiratory syndrome coronavirus gene 7 products contribute to virus-induced apoptosis. / Schaecher, Scott R.; Touchette, Erin; Schriewer, Jill; Buller, R. Mark; Pekosz, Andrew.

In: Journal of Virology, Vol. 81, No. 20, 10.2007, p. 11054-11068.

Research output: Contribution to journalArticle

Schaecher, Scott R. ; Touchette, Erin ; Schriewer, Jill ; Buller, R. Mark ; Pekosz, Andrew. / Severe acute respiratory syndrome coronavirus gene 7 products contribute to virus-induced apoptosis. In: Journal of Virology. 2007 ; Vol. 81, No. 20. pp. 11054-11068.
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