Seven helix chemoattractant receptors transiently stimulate mitogen-activated protein kinase in Dictyostelium: Role of heterotrimeric G proteins

Mineko Maeda, Laurence Aubry, Robert Insall, Chris Gaskins, Peter N Devreotes, Richard A. Firtel

Research output: Contribution to journalArticle

Abstract

Mitogen-activated protein (MAP) kinases are involved in controlling a cell's responses to a variety of stimuli and can be activated by both protein tyrosine kinase and G protein-coupled receptors. It was shown previously that Dictyostelium MAP kinase ERK2 is required for normal activation of adenylyl cyclase and erk2 null cells are aggregation-deficient. In this manuscript, we show that the Dictyostelium MAP kinase ERK2 is rapidly and transiently activated in response to the chemoattractant cAMP. This response requires cAMP receptors, but is independent of the coupled Gα2 subunit and the only known Gβ subunit. These data indicate that ligand-mediated receptor activation of adenylyl cyclase requires two receptor-dependent pathways, one of which requires heterotrimeric G proteins, including Gα2 and the only known Gβ subunit, and the second of which requires ERK2. Our results suggest that ERK2 may be activated by a novel receptor-mediated pathway.

Original languageEnglish (US)
Pages (from-to)3351-3354
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number7
StatePublished - Feb 16 1996

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Formyl Peptide Receptor
Heterotrimeric GTP-Binding Proteins
Dictyostelium
Mitogen-Activated Protein Kinases
Adenylyl Cyclases
Chemical activation
Cyclic AMP Receptors
Cyclic GMP-Dependent Protein Kinases
Null Lymphocytes
Cell Aggregation
Chemotactic Factors
G-Protein-Coupled Receptors
Protein-Tyrosine Kinases
Agglomeration
Ligands

ASJC Scopus subject areas

  • Biochemistry

Cite this

Seven helix chemoattractant receptors transiently stimulate mitogen-activated protein kinase in Dictyostelium : Role of heterotrimeric G proteins. / Maeda, Mineko; Aubry, Laurence; Insall, Robert; Gaskins, Chris; Devreotes, Peter N; Firtel, Richard A.

In: Journal of Biological Chemistry, Vol. 271, No. 7, 16.02.1996, p. 3351-3354.

Research output: Contribution to journalArticle

Maeda, Mineko ; Aubry, Laurence ; Insall, Robert ; Gaskins, Chris ; Devreotes, Peter N ; Firtel, Richard A. / Seven helix chemoattractant receptors transiently stimulate mitogen-activated protein kinase in Dictyostelium : Role of heterotrimeric G proteins. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 7. pp. 3351-3354.
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