Serum neurogranin measurement as a biomarker of acute traumatic brain injury

Research output: Contribution to journalArticle

Abstract

Objectives: Neurogranin (NRGN) is a small neuronal protein that plays an important role in synaptic signaling by regulating calmodulin (CaM) availability. In this study, we developed an ELISA to measure NRGN quantitatively in serum samples from a cohort of acute traumatic brain injury (TBI) patients and a non-TBI control cohort, and explored the potential value of NRGN as a circulating biomarker for TBI. Design and methods: Recombinant His-NRGN protein was used to develop mouse monoclonal capture and rabbit polyclonal detection antibodies, and they were used to develop a sandwich ELISA. After validation, we used this ELISA to measure serum samples from a cohort of typical adult acute TBI patients (N= 76 TBI cases) and non-TBI control patients (N= 150 controls). Results: The NRGN ELISA lower limit of detection was 0.055. ng/mL, lower limit of quantification was 0.2. ng/mL, and interassay CVs were ≤. 10.7%. The average recovery was 99.9% (range from 97.2-102%). Serum NRGN concentrations in TBI cases were significantly higher than in controls (median values were 0.18. ng/mL vs. 0.02. ng/mL, p

Original languageEnglish (US)
Pages (from-to)843-848
Number of pages6
JournalClinical Biochemistry
Volume48
Issue number13-14
DOIs
StatePublished - Sep 1 2015

Fingerprint

Neurogranin
Biomarkers
Brain Injuries
Brain
Serum
Enzyme-Linked Immunosorbent Assay
Calmodulin
Limit of Detection
Traumatic Brain Injury
Proteins
Rabbits
Availability
Antibodies
Recovery

Keywords

  • Biomarker
  • ELISA
  • Neurogranin
  • Traumatic brain injury

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Serum neurogranin measurement as a biomarker of acute traumatic brain injury. / Yang, Jun; Korley, Frederick; Dai, Min; Everett, Allen D.

In: Clinical Biochemistry, Vol. 48, No. 13-14, 01.09.2015, p. 843-848.

Research output: Contribution to journalArticle

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