Serum biomarkers for detection of head and neck squamous cell carcinoma

Wojciech K. Mydlarz; Patrick T. Hennessey; Hao Wang; Andre Lopez Carvalho; Joseph A. Califano

doi:10.1002/hed.23842

Serum biomarkers for detection of head and neck squamous cell carcinoma

Wojciech K. Mydlarz, Patrick T. Hennessey, Hao Wang, Andre Lopez Carvalho, Joseph A. Califano

School of Medicine

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Background Detection of hypermethylated circulating tumor DNA has the potential to be a minimally invasive, low cost, and reproducible method for cancer detection. Methods We evaluated serum from 100 patients with known head and neck squamous cell carcinoma (HNSCC) and 50 healthy control patients for 3 previously described methylation targets, endothelin receptor type B (EDNRB), cyclin-dependent kinase inhibitor 2A (CDKN2A or p16), and deleted in colorectal carcinoma (DCC), using quantitative methylation specific polymerase chain reaction (qMSPCR). Results EDNRB hypermethylation was identified in the serum of 10% of the patients with HNSCC but in none of the control patients. DCC hypermethylation was detected in 2 serum samples from patients with cancer that also amplified EDNRB and one of these samples also had p16 hypermethylation. EDNRB hypermethylation was statistically significant by Fisher's exact test (p =.03) when comparing HNSCC to controls. Conclusions Serum EDNRB hypermethylation is a highly specific but not sensitive serum biomarker for HNSCC.

Original language	English (US)
Pages (from-to)	9-14
Number of pages	6
Journal	Head and Neck
Volume	38
Issue number	1
DOIs	https://doi.org/10.1002/hed.23842
State	Published - Jan 1 2016

Keywords

epigenetics
head and neck cancer
head and neck squamous cell carcinoma (HNSCC)
hypermethylation
screening

ASJC Scopus subject areas

Otorhinolaryngology

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10.1002/hed.23842

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title = "Serum biomarkers for detection of head and neck squamous cell carcinoma",

abstract = "Background Detection of hypermethylated circulating tumor DNA has the potential to be a minimally invasive, low cost, and reproducible method for cancer detection. Methods We evaluated serum from 100 patients with known head and neck squamous cell carcinoma (HNSCC) and 50 healthy control patients for 3 previously described methylation targets, endothelin receptor type B (EDNRB), cyclin-dependent kinase inhibitor 2A (CDKN2A or p16), and deleted in colorectal carcinoma (DCC), using quantitative methylation specific polymerase chain reaction (qMSPCR). Results EDNRB hypermethylation was identified in the serum of 10% of the patients with HNSCC but in none of the control patients. DCC hypermethylation was detected in 2 serum samples from patients with cancer that also amplified EDNRB and one of these samples also had p16 hypermethylation. EDNRB hypermethylation was statistically significant by Fisher's exact test (p =.03) when comparing HNSCC to controls. Conclusions Serum EDNRB hypermethylation is a highly specific but not sensitive serum biomarker for HNSCC.",

keywords = "epigenetics, head and neck cancer, head and neck squamous cell carcinoma (HNSCC), hypermethylation, screening",

author = "Mydlarz, {Wojciech K.} and Hennessey, {Patrick T.} and Hao Wang and Carvalho, {Andre Lopez} and Califano, {Joseph A.}",

note = "Funding Information: Dr. Califano is supported by an NCI/NIDCR SPORE (5P50CA096784-05) and NIDCR Challenge Grant (RC1DE020324) and Drs. Mydlarz and Hennessey are funded in part by a NIH T32 Research Training Grant (T32DC000027). Publisher Copyright: {\textcopyright} 2015 Wiley Periodicals, Inc.",

year = "2016",

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doi = "10.1002/hed.23842",

language = "English (US)",

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T1 - Serum biomarkers for detection of head and neck squamous cell carcinoma

AU - Mydlarz, Wojciech K.

AU - Hennessey, Patrick T.

AU - Wang, Hao

AU - Carvalho, Andre Lopez

AU - Califano, Joseph A.

N1 - Funding Information: Dr. Califano is supported by an NCI/NIDCR SPORE (5P50CA096784-05) and NIDCR Challenge Grant (RC1DE020324) and Drs. Mydlarz and Hennessey are funded in part by a NIH T32 Research Training Grant (T32DC000027). Publisher Copyright: © 2015 Wiley Periodicals, Inc.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Background Detection of hypermethylated circulating tumor DNA has the potential to be a minimally invasive, low cost, and reproducible method for cancer detection. Methods We evaluated serum from 100 patients with known head and neck squamous cell carcinoma (HNSCC) and 50 healthy control patients for 3 previously described methylation targets, endothelin receptor type B (EDNRB), cyclin-dependent kinase inhibitor 2A (CDKN2A or p16), and deleted in colorectal carcinoma (DCC), using quantitative methylation specific polymerase chain reaction (qMSPCR). Results EDNRB hypermethylation was identified in the serum of 10% of the patients with HNSCC but in none of the control patients. DCC hypermethylation was detected in 2 serum samples from patients with cancer that also amplified EDNRB and one of these samples also had p16 hypermethylation. EDNRB hypermethylation was statistically significant by Fisher's exact test (p =.03) when comparing HNSCC to controls. Conclusions Serum EDNRB hypermethylation is a highly specific but not sensitive serum biomarker for HNSCC.

AB - Background Detection of hypermethylated circulating tumor DNA has the potential to be a minimally invasive, low cost, and reproducible method for cancer detection. Methods We evaluated serum from 100 patients with known head and neck squamous cell carcinoma (HNSCC) and 50 healthy control patients for 3 previously described methylation targets, endothelin receptor type B (EDNRB), cyclin-dependent kinase inhibitor 2A (CDKN2A or p16), and deleted in colorectal carcinoma (DCC), using quantitative methylation specific polymerase chain reaction (qMSPCR). Results EDNRB hypermethylation was identified in the serum of 10% of the patients with HNSCC but in none of the control patients. DCC hypermethylation was detected in 2 serum samples from patients with cancer that also amplified EDNRB and one of these samples also had p16 hypermethylation. EDNRB hypermethylation was statistically significant by Fisher's exact test (p =.03) when comparing HNSCC to controls. Conclusions Serum EDNRB hypermethylation is a highly specific but not sensitive serum biomarker for HNSCC.

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Serum biomarkers for detection of head and neck squamous cell carcinoma

Abstract

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