Serum antibodies to human papillomavirus 16 proteins in women from Brazil with invasive cervical carcinoma

Yeping Sun, José Eluf-Neto, F. Xavier Bosch, Nubia Muñoz, Jan M.M. Walboomers, Chris J.L.M. Meijer, Keerti V. Shah, Barbara Clayman, Raphael P. Viscidi

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Serum samples from 194 cases and 217 controls participating in a case- control study of invasive cervical cancer in Brazil were examined for antibodies to human papillomavirus (HPV) 16 virus-like particles (VLPs) by ELISA. The prevalence of antibody in cases and controls was 47.4 versus 24.4% (P < 0.001). The prevalence was higher in women who had HPV-16 DNA in the genital tract (54.2%) than in those with other HPVs (36.8%) or no HPVs (44.8%), but the differences were not statistically significant. Among cases and controls, HPV-16 VLP antibodies were associated with a greater number of lifetime sexual partners (χ2 for trend, P < 0.001). Among controls, age was inversely associated with HPV-16 VLP seroreactivity (χ2 for trend, P = 0.019). The sera were previously tested for antibodies to HPV-16 E6 and E7 oncoproteins; there was no correlation between antibody titers to HPV-16 E6 or E7 and VLPs. The HPV-16 serological assays were compared as screening tests for invasive cervical cancer. The sensitivity and specificity estimates were 47.4 and 75.6% for HPV-16 VLP serology, 63.4 and 89.9% for either HPV-16 E6 or E7 serology, and 53.6 and 93.6% for high titers of either HPV-16 E6 or E7 or VLP antibodies. The utility of HPV-16 VLP ELISA as a screening test for invasive cervical cancer is limited by a high seroprevalence in women with probable prior exposure to HVP 16 but without disease.

Original languageEnglish (US)
Pages (from-to)935-940
Number of pages6
JournalCancer Epidemiology Biomarkers and Prevention
Issue number10
StatePublished - Oct 1999

ASJC Scopus subject areas

  • Epidemiology
  • Oncology


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