Serum albumin adducts in the molecular epidemiology of aflatoxin carcinogenesis: Correlation with aflatoxin b1 intake and urinary excretion of aflatoxin m1

Liang Shang Gan, Paul L. Skipper, Xiaocong Peng, John D. Groopman, Jun shi Chen, Gerald N. Wogan, Steven R. Tannenbaum

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Abstract

Aflatoxin-serum albumin adducts in the blood of 42 residents of Guangxi Province, People's Republic of China, were determined and compared with intake of aflatoxin B1 (AFB1) and excretion of aflatoxin M1 (AFM1) in urine. Blood specimens were obtained during the same period that urine was collected and that diet was sampled. Serum albumin was isolated from blood by affinity chromatography on Reactive Blue 2-Sepharose and subjected to enzymatic proteolysis using Pronase. Immunoreactive products were purified by immunoaffinity chromatography and quantified by competitive radioimmunoassay. A highly significant correlation (r = 0.60, P < 0.00003) of adduct level with AFM, excretion was observed. An equally highly significant correlation of adduct level with intake (r = 0.69, P < 0.000001) was also observed. From the slope of the regression line for adduct level as a function of intake, it was calculated that 1.4-2.3% of ingested AFB1 becomes covalently bound to serum albumin, a value very similar to that observed when rats are administered AFB1.

Original languageEnglish (US)
Pages (from-to)1323-1325
Number of pages3
JournalCarcinogenesis
Volume9
Issue number7
DOIs
StatePublished - Jul 1 1988
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research

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