Serum albumin adducts in the molecular epidemiology of aflatoxin carcinogenesis: Correlation with aflatoxin b1 intake and urinary excretion of aflatoxin m1

Liang Shang Gan, Paul L. Skipper, Xiaocong Peng, John Davis Groopman, Jun shi Chen, Gerald N. Wogan, Steven R. Tannenbaum

Research output: Contribution to journalArticle

Abstract

Aflatoxin-serum albumin adducts in the blood of 42 residents of Guangxi Province, People's Republic of China, were determined and compared with intake of aflatoxin B1 (AFB1) and excretion of aflatoxin M1 (AFM1) in urine. Blood specimens were obtained during the same period that urine was collected and that diet was sampled. Serum albumin was isolated from blood by affinity chromatography on Reactive Blue 2-Sepharose and subjected to enzymatic proteolysis using Pronase. Immunoreactive products were purified by immunoaffinity chromatography and quantified by competitive radioimmunoassay. A highly significant correlation (r = 0.60, P <0.00003) of adduct level with AFM, excretion was observed. An equally highly significant correlation of adduct level with intake (r = 0.69, P <0.000001) was also observed. From the slope of the regression line for adduct level as a function of intake, it was calculated that 1.4-2.3% of ingested AFB1 becomes covalently bound to serum albumin, a value very similar to that observed when rats are administered AFB1.

Original languageEnglish (US)
Pages (from-to)1323-1325
Number of pages3
JournalCarcinogenesis
Volume9
Issue number7
DOIs
StatePublished - Jul 1988
Externally publishedYes

Fingerprint

Aflatoxin M1
Aflatoxins
Aflatoxin B1
Molecular Epidemiology
Carcinogenesis
Epidemiology
Serum Albumin
Blood
Cibacron Blue F 3GA
Chromatography
Urine
Regression line
Pronase
Affinity Chromatography
Proteolysis
Affine transformation
Radioimmunoassay
China
Slope
Affinity chromatography

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience
  • Cancer Research

Cite this

Serum albumin adducts in the molecular epidemiology of aflatoxin carcinogenesis : Correlation with aflatoxin b1 intake and urinary excretion of aflatoxin m1. / Gan, Liang Shang; Skipper, Paul L.; Peng, Xiaocong; Groopman, John Davis; Chen, Jun shi; Wogan, Gerald N.; Tannenbaum, Steven R.

In: Carcinogenesis, Vol. 9, No. 7, 07.1988, p. 1323-1325.

Research output: Contribution to journalArticle

Gan, Liang Shang ; Skipper, Paul L. ; Peng, Xiaocong ; Groopman, John Davis ; Chen, Jun shi ; Wogan, Gerald N. ; Tannenbaum, Steven R. / Serum albumin adducts in the molecular epidemiology of aflatoxin carcinogenesis : Correlation with aflatoxin b1 intake and urinary excretion of aflatoxin m1. In: Carcinogenesis. 1988 ; Vol. 9, No. 7. pp. 1323-1325.
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abstract = "Aflatoxin-serum albumin adducts in the blood of 42 residents of Guangxi Province, People's Republic of China, were determined and compared with intake of aflatoxin B1 (AFB1) and excretion of aflatoxin M1 (AFM1) in urine. Blood specimens were obtained during the same period that urine was collected and that diet was sampled. Serum albumin was isolated from blood by affinity chromatography on Reactive Blue 2-Sepharose and subjected to enzymatic proteolysis using Pronase. Immunoreactive products were purified by immunoaffinity chromatography and quantified by competitive radioimmunoassay. A highly significant correlation (r = 0.60, P <0.00003) of adduct level with AFM, excretion was observed. An equally highly significant correlation of adduct level with intake (r = 0.69, P <0.000001) was also observed. From the slope of the regression line for adduct level as a function of intake, it was calculated that 1.4-2.3{\%} of ingested AFB1 becomes covalently bound to serum albumin, a value very similar to that observed when rats are administered AFB1.",
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AB - Aflatoxin-serum albumin adducts in the blood of 42 residents of Guangxi Province, People's Republic of China, were determined and compared with intake of aflatoxin B1 (AFB1) and excretion of aflatoxin M1 (AFM1) in urine. Blood specimens were obtained during the same period that urine was collected and that diet was sampled. Serum albumin was isolated from blood by affinity chromatography on Reactive Blue 2-Sepharose and subjected to enzymatic proteolysis using Pronase. Immunoreactive products were purified by immunoaffinity chromatography and quantified by competitive radioimmunoassay. A highly significant correlation (r = 0.60, P <0.00003) of adduct level with AFM, excretion was observed. An equally highly significant correlation of adduct level with intake (r = 0.69, P <0.000001) was also observed. From the slope of the regression line for adduct level as a function of intake, it was calculated that 1.4-2.3% of ingested AFB1 becomes covalently bound to serum albumin, a value very similar to that observed when rats are administered AFB1.

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