Serratia liquefaciens bloodstream infections from contamination of epoetin alfa at a hemodialysis center

Lisa A. Grohskopf, Virginia R. Roth, Daniel Feikin, Matthew J. Arduino, Loretta A. Carson, Jerome I. Tokars, Stacey C. Holt, Bette J. Jensen, Richard E. Hoffman, William R. Jarvis

Research output: Contribution to journalArticle

Abstract

Background: In one month, 10 Serratia liquefaciens bloodstream infections and 6 pyrogenic reactions occurred in outpatients at a hemodialysis center. Methods: We performed a cohort study of all hemodialysis sessions on days that staff members reported S. liquefaciens bloodstream infections or pyrogenic reactions. We reviewed procedures and cultured samples of water, medications, soaps, and hand lotions and swabs from the hands of personnel. Results: We analyzed 208 sessions involving 48 patients. In 12 sessions, patients had S. liquefaciens bloodstream infections, and in 8, patients had pyrogenie reactions without bloodstream infection. Sessions with infections or reactions were associated with higher median doses of epoetin alfa than the 188 other sessions (6500 vs. 4000 U, P=0.03) and were more common during afternoon or evening shifts than morning shifts (P=0.03). Sessions with infections or reactions were associated with doses of epoetin alfa of more than 4000 U (multivariate odds ratio, 4.0; 95 percent confidence interval, 1.3 to 12.3). A review of procedures revealed that preservative-free, single-use vials of epoetin alfa were punctured multiple times, and residual epoetin alfa from multiple vials was pooled and administered to patients. S. liquefaciens was isolated from pooled epoetin alfa, empty vials of epoetin alfa, antibacterial soap, and hand lotion. All the isolates were identical by pulsed-field gel electrophoresis. After the practice of pooling epoetin alfa was discontinued and the contaminated soap and lotion were replaced, no further S. liquefaciens bloodstream infections or pyrogenic reactions occurred at this hemodialysis facility. Conclusions: Puncturing single-use vials multiple times and pooling preservative-free epoetin alfa caused this outbreak of bloodstream infections in a hemodialysis unit. To prevent similar outbreaks, dialysis units should use medication vials containing the doses most appropriate to their clinical needs.

Original languageEnglish (US)
Pages (from-to)1491-1497
Number of pages7
JournalNew England Journal of Medicine
Volume344
Issue number20
DOIs
StatePublished - May 17 2001
Externally publishedYes

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Epoetin Alfa
Serratia liquefaciens
Renal Dialysis
Infection
Soaps
Hand
Disease Outbreaks
Pulsed Field Gel Electrophoresis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Grohskopf, L. A., Roth, V. R., Feikin, D., Arduino, M. J., Carson, L. A., Tokars, J. I., ... Jarvis, W. R. (2001). Serratia liquefaciens bloodstream infections from contamination of epoetin alfa at a hemodialysis center. New England Journal of Medicine, 344(20), 1491-1497. https://doi.org/10.1056/NEJM200105173442001

Serratia liquefaciens bloodstream infections from contamination of epoetin alfa at a hemodialysis center. / Grohskopf, Lisa A.; Roth, Virginia R.; Feikin, Daniel; Arduino, Matthew J.; Carson, Loretta A.; Tokars, Jerome I.; Holt, Stacey C.; Jensen, Bette J.; Hoffman, Richard E.; Jarvis, William R.

In: New England Journal of Medicine, Vol. 344, No. 20, 17.05.2001, p. 1491-1497.

Research output: Contribution to journalArticle

Grohskopf, LA, Roth, VR, Feikin, D, Arduino, MJ, Carson, LA, Tokars, JI, Holt, SC, Jensen, BJ, Hoffman, RE & Jarvis, WR 2001, 'Serratia liquefaciens bloodstream infections from contamination of epoetin alfa at a hemodialysis center', New England Journal of Medicine, vol. 344, no. 20, pp. 1491-1497. https://doi.org/10.1056/NEJM200105173442001
Grohskopf, Lisa A. ; Roth, Virginia R. ; Feikin, Daniel ; Arduino, Matthew J. ; Carson, Loretta A. ; Tokars, Jerome I. ; Holt, Stacey C. ; Jensen, Bette J. ; Hoffman, Richard E. ; Jarvis, William R. / Serratia liquefaciens bloodstream infections from contamination of epoetin alfa at a hemodialysis center. In: New England Journal of Medicine. 2001 ; Vol. 344, No. 20. pp. 1491-1497.
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abstract = "Background: In one month, 10 Serratia liquefaciens bloodstream infections and 6 pyrogenic reactions occurred in outpatients at a hemodialysis center. Methods: We performed a cohort study of all hemodialysis sessions on days that staff members reported S. liquefaciens bloodstream infections or pyrogenic reactions. We reviewed procedures and cultured samples of water, medications, soaps, and hand lotions and swabs from the hands of personnel. Results: We analyzed 208 sessions involving 48 patients. In 12 sessions, patients had S. liquefaciens bloodstream infections, and in 8, patients had pyrogenie reactions without bloodstream infection. Sessions with infections or reactions were associated with higher median doses of epoetin alfa than the 188 other sessions (6500 vs. 4000 U, P=0.03) and were more common during afternoon or evening shifts than morning shifts (P=0.03). Sessions with infections or reactions were associated with doses of epoetin alfa of more than 4000 U (multivariate odds ratio, 4.0; 95 percent confidence interval, 1.3 to 12.3). A review of procedures revealed that preservative-free, single-use vials of epoetin alfa were punctured multiple times, and residual epoetin alfa from multiple vials was pooled and administered to patients. S. liquefaciens was isolated from pooled epoetin alfa, empty vials of epoetin alfa, antibacterial soap, and hand lotion. All the isolates were identical by pulsed-field gel electrophoresis. After the practice of pooling epoetin alfa was discontinued and the contaminated soap and lotion were replaced, no further S. liquefaciens bloodstream infections or pyrogenic reactions occurred at this hemodialysis facility. Conclusions: Puncturing single-use vials multiple times and pooling preservative-free epoetin alfa caused this outbreak of bloodstream infections in a hemodialysis unit. To prevent similar outbreaks, dialysis units should use medication vials containing the doses most appropriate to their clinical needs.",
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AU - Grohskopf, Lisa A.

AU - Roth, Virginia R.

AU - Feikin, Daniel

AU - Arduino, Matthew J.

AU - Carson, Loretta A.

AU - Tokars, Jerome I.

AU - Holt, Stacey C.

AU - Jensen, Bette J.

AU - Hoffman, Richard E.

AU - Jarvis, William R.

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N2 - Background: In one month, 10 Serratia liquefaciens bloodstream infections and 6 pyrogenic reactions occurred in outpatients at a hemodialysis center. Methods: We performed a cohort study of all hemodialysis sessions on days that staff members reported S. liquefaciens bloodstream infections or pyrogenic reactions. We reviewed procedures and cultured samples of water, medications, soaps, and hand lotions and swabs from the hands of personnel. Results: We analyzed 208 sessions involving 48 patients. In 12 sessions, patients had S. liquefaciens bloodstream infections, and in 8, patients had pyrogenie reactions without bloodstream infection. Sessions with infections or reactions were associated with higher median doses of epoetin alfa than the 188 other sessions (6500 vs. 4000 U, P=0.03) and were more common during afternoon or evening shifts than morning shifts (P=0.03). Sessions with infections or reactions were associated with doses of epoetin alfa of more than 4000 U (multivariate odds ratio, 4.0; 95 percent confidence interval, 1.3 to 12.3). A review of procedures revealed that preservative-free, single-use vials of epoetin alfa were punctured multiple times, and residual epoetin alfa from multiple vials was pooled and administered to patients. S. liquefaciens was isolated from pooled epoetin alfa, empty vials of epoetin alfa, antibacterial soap, and hand lotion. All the isolates were identical by pulsed-field gel electrophoresis. After the practice of pooling epoetin alfa was discontinued and the contaminated soap and lotion were replaced, no further S. liquefaciens bloodstream infections or pyrogenic reactions occurred at this hemodialysis facility. Conclusions: Puncturing single-use vials multiple times and pooling preservative-free epoetin alfa caused this outbreak of bloodstream infections in a hemodialysis unit. To prevent similar outbreaks, dialysis units should use medication vials containing the doses most appropriate to their clinical needs.

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