Serotyping of Streptococcus pneumoniae by coagglutination with 12 pooled antisera

M. K. Lalitha, Kurien Thomas, R. Satish Kumar, Mark C. Steinhoff, N. K. Arora, Bimal Das, Dipty Jain, Vandana Agarwal, Shally Awasti, Amita Jain, N. Deivanayagam, R. Manjula, P. Raghupathy, Thomas Cherian, Reba Kanungo

Research output: Contribution to journalArticle

Abstract

We report on the performance of a recently introduced commercial chessboard method using 12 antisera, in comparison with that of the 55- antiserum panel used in determining the serogroups and types (SGTs) of Streptococcus pneumoniae, both of which were carried out by a coagglutination technique. Of a total of 150 strains of S. pneumoniae studied, 135 (90%) belonged to the SGTs represented in the 23-valent pneumococcal vaccine; of these, 130 (96.3%) were identified as the same SGTs by both typing methods. The remaining five strains showed cross-reactivity with more than two pools by the chessboard method, but could be assigned to a single SGT by the Quellung test. The 96.3% concordance of the chessboard method suggests it can be adopted for determination of the SGTs of S. pneumoniae in laboratories.

Original languageEnglish (US)
Pages (from-to)263-265
Number of pages3
JournalJournal of clinical microbiology
Volume37
Issue number1
StatePublished - Jan 1 1999

ASJC Scopus subject areas

  • Microbiology (medical)

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    Lalitha, M. K., Thomas, K., Satish Kumar, R., Steinhoff, M. C., Arora, N. K., Das, B., Jain, D., Agarwal, V., Awasti, S., Jain, A., Deivanayagam, N., Manjula, R., Raghupathy, P., Cherian, T., & Kanungo, R. (1999). Serotyping of Streptococcus pneumoniae by coagglutination with 12 pooled antisera. Journal of clinical microbiology, 37(1), 263-265.