Serotyping of Streptococcus pneumoniae by agglutination assays: A cost-effective technique for developing countries

M. K. Lalitha, R. Pai, T. J. John, K. Thomas, M. V. Jesudason, K. N. Brahmadathan, G. Sridharan, M. C. Steinhoff

Research output: Contribution to journalArticle

Abstract

There is a need for additional data on the distribution of pneumococcal serotypes in developing countries. We report the use of a coagglutination (COA) and a latex agglutination (LA) test for serotyping Streptococcus pneumoniae which were evaluated using 114 clinical isolates in Vellore, India. In tests to serotype 30 fresh isolates of pneumococci from meningitis (8 isolates), bacteraemia/septicaemia (21 isolates) and peritonitis (1 isolate) cases, there was complete concordance among the three methods. An additional 20 isolates (11 from cerebrospinal fluid and 9 from blood cultures) were serotyped using both LA and COA, with full agreement between the results. With a further 30 isolates, there was 93% concordance for the COA types with serotypes assigned by a WHO reference laboratory. The COA and LA serotyping results were equivalent in accuracy to those obtained using quellung serotyping. Both these agglutination tests are rapid, valid, and relatively cheap, and with appropriate validation by reference laboratories they could be more widely used in developing countries to obtain local and regional data on pneumococcal serotype distribution.

Original languageEnglish (US)
Pages (from-to)387-390
Number of pages4
JournalBulletin of the World Health Organization
Volume74
Issue number4
StatePublished - 1996

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Serotyping
Agglutination
Streptococcus pneumoniae
Developing Countries
Costs and Cost Analysis
Latex
Latex Fixation Tests
Agglutination Tests
Bacteremia
Peritonitis
Meningitis
Cerebrospinal Fluid
India
Sepsis
Serogroup

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Lalitha, M. K., Pai, R., John, T. J., Thomas, K., Jesudason, M. V., Brahmadathan, K. N., ... Steinhoff, M. C. (1996). Serotyping of Streptococcus pneumoniae by agglutination assays: A cost-effective technique for developing countries. Bulletin of the World Health Organization, 74(4), 387-390.

Serotyping of Streptococcus pneumoniae by agglutination assays : A cost-effective technique for developing countries. / Lalitha, M. K.; Pai, R.; John, T. J.; Thomas, K.; Jesudason, M. V.; Brahmadathan, K. N.; Sridharan, G.; Steinhoff, M. C.

In: Bulletin of the World Health Organization, Vol. 74, No. 4, 1996, p. 387-390.

Research output: Contribution to journalArticle

Lalitha, MK, Pai, R, John, TJ, Thomas, K, Jesudason, MV, Brahmadathan, KN, Sridharan, G & Steinhoff, MC 1996, 'Serotyping of Streptococcus pneumoniae by agglutination assays: A cost-effective technique for developing countries', Bulletin of the World Health Organization, vol. 74, no. 4, pp. 387-390.
Lalitha, M. K. ; Pai, R. ; John, T. J. ; Thomas, K. ; Jesudason, M. V. ; Brahmadathan, K. N. ; Sridharan, G. ; Steinhoff, M. C. / Serotyping of Streptococcus pneumoniae by agglutination assays : A cost-effective technique for developing countries. In: Bulletin of the World Health Organization. 1996 ; Vol. 74, No. 4. pp. 387-390.
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