Serotyping of human group A rotavirus with oligonucleotide probes

Orntipa Sethabutr, Leanne E. Uniocomb, Ian H. Holmes, David N. Taylor, Ruth F. Bishop, Peter Echeverria

Research output: Contribution to journalArticlepeer-review

Abstract

Rotaviruses(RV) in stools of children with diarrhea in Thailand were serotyped by monoclonal enzyme immunoassay(MEIA), and RNA extracted from these specimens were tested for hybridization with oligonucleotides constructed from the nucleotide sequences of VP7 of human serotypes 1, 2, 3, and 4. Of 178 specimens that contained RV as identified with a monoclonal antibody to group A RV, 84% (149/178) hybridized with serotype-specific oligonucleotides, and 42% (74/178) were serotyped by MEIA (P <.001). Of the 74 specimens that were serotyped by MEIA, 92% (35/38) of type 1, 97% (34/35) of type 2, and the one type 4 RV hybridized with the HuGlAc, HuG2Ac, and HuG4Ac oligonucleotides, respectively. RV strains identified in children in Thailand in 1987 and 1988 to which a serotype could be assigned by either method were either type 1, type 2, or, less often, type 4. Testing RV for hybridization with oligonucleotides for genes encoding VP7 is an alternate method of determining RV serotypes.

Original languageEnglish (US)
Pages (from-to)368-372
Number of pages5
JournalJournal of Infectious Diseases
Volume162
Issue number2
DOIs
StatePublished - Aug 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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