Serotyping isolates of Anaplasma phagocytophilum by using monoclonal antibodies

Hisashi Inokuma, Philippe Brouqui, J. Stephen Dumler, Didier Raoult

Research output: Contribution to journalArticlepeer-review

Abstract

Ten mouse monoclonal antibodies (MAbs) that react with Anaplasma phagocytophilum (the human granulocytic ehrlichiosis agent) Webster isolates were developed. Seven different isolates of A. phagocytophilum were subtyped with these MAbs. Western blot analysis revealed that these MAbs reacted mainly with 41- to 46-kDa Msp2 proteins. Six MAbs reacted with all isolates. Four other MAbs reacted with human isolates from Wisconsin, but not with human isolates from New York or with animal isolates. Three different serotypes were identified. These features may lead to the development of other specific MAbs in order to provide tools for antigenic characterization of human isolates of A. phagocytophilum.

Original languageEnglish (US)
Pages (from-to)969-972
Number of pages4
JournalClinical and Diagnostic Laboratory Immunology
Volume10
Issue number5
DOIs
StatePublished - Sep 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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