Serotypes of Streptococcus pneumoniae causing meningitis in Southern India. Use of new direct latex agglutination antigen detection tests in cerebrospinal fluid

Gopalan Sridharan, T. Jacob John, M. K. Lalitha, Lee H. Harrison, Mark C. Steinhoff

Research output: Contribution to journalArticlepeer-review

Abstract

The utility of a new latex agglutination (LA) test to directly determine serotypes of Streptococcus pneumoniae in cerebrospinal fluid (CSF) was assessed in prospective evaluation at a referral hospital in southern India. Samples from 18 ill patients with Gram-positive organisms in CSF were tested. The presence of C polysaccharide or specific serotype antigen (types 1, 3, 4, 5, 6, 12, 14, 18, 19, and 23) of S. pneumoniae was detected by slide LA test. Pneumococcal antigen was detected in 17 (94%) of 18 CSF specimens; in 14 (78%) the serotype was determined directly. Serotypes 1, 5, 6, 19, 23, 7, 10, 34, and 38 were found in these patients. A quellung test of the cultured isolates confirmed the serotypes. Information regarding the serotype distribution of pneumococci in varied geographic locations is important for the design and evaluation of pneumococcal vaccines. The slide LA tests seemed useful in detecting S. pneumoniae antigens and in determining the serotype, and have promise in simplifying the gathering of serotype data.

Original languageEnglish (US)
Pages (from-to)211-214
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume18
Issue number4
DOIs
StatePublished - Apr 1994

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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