Serotonin in human lumbar cerebrospinal fluid: A reassessment

George M. Anderson, Ivan N. Mefford, Teresa J. Tolliver, Mark A Riddle, Dave M. Ocame, James F. Leckman, Donald J. Cohen

Research output: Contribution to journalArticle

Abstract

An inter-laboratory comparison study was carried out in order to ascertain mean levels of serotonin (5-HT) in human lumbar cerebrospinal fluid (CSF). Analyses were performed using high performance liquid chromatogrophy (HPLC) coupled with either electrochemical (LC-EC) or fluorometric (LC-F) detection. with the detection limits obtained (7-8 pg/ml for LC-EC, 7-15 pg/ml for LC-F) 5-HT was not usually detected in human lumbar CSF. The findings indicate that the true mean concentration of CSF 5-HT is less than 10 pg/ml. This upper limit is substantially lower than all previous reports of 5-HT concentrations in normal lumbar CSF. The extremely low concentrations of 5-HT present in CSF make it unlikely that CSF 5-HT will be of clinical utility in assessing central serotonergic function.

Original languageEnglish (US)
Pages (from-to)247-255
Number of pages9
JournalLife Sciences
Volume46
Issue number4
DOIs
StatePublished - 1990
Externally publishedYes

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Cerebrospinal fluid
Cerebrospinal Fluid
Serotonin
Limit of Detection
Liquids

ASJC Scopus subject areas

  • Pharmacology

Cite this

Anderson, G. M., Mefford, I. N., Tolliver, T. J., Riddle, M. A., Ocame, D. M., Leckman, J. F., & Cohen, D. J. (1990). Serotonin in human lumbar cerebrospinal fluid: A reassessment. Life Sciences, 46(4), 247-255. https://doi.org/10.1016/0024-3205(90)90030-U

Serotonin in human lumbar cerebrospinal fluid : A reassessment. / Anderson, George M.; Mefford, Ivan N.; Tolliver, Teresa J.; Riddle, Mark A; Ocame, Dave M.; Leckman, James F.; Cohen, Donald J.

In: Life Sciences, Vol. 46, No. 4, 1990, p. 247-255.

Research output: Contribution to journalArticle

Anderson, GM, Mefford, IN, Tolliver, TJ, Riddle, MA, Ocame, DM, Leckman, JF & Cohen, DJ 1990, 'Serotonin in human lumbar cerebrospinal fluid: A reassessment', Life Sciences, vol. 46, no. 4, pp. 247-255. https://doi.org/10.1016/0024-3205(90)90030-U
Anderson GM, Mefford IN, Tolliver TJ, Riddle MA, Ocame DM, Leckman JF et al. Serotonin in human lumbar cerebrospinal fluid: A reassessment. Life Sciences. 1990;46(4):247-255. https://doi.org/10.1016/0024-3205(90)90030-U
Anderson, George M. ; Mefford, Ivan N. ; Tolliver, Teresa J. ; Riddle, Mark A ; Ocame, Dave M. ; Leckman, James F. ; Cohen, Donald J. / Serotonin in human lumbar cerebrospinal fluid : A reassessment. In: Life Sciences. 1990 ; Vol. 46, No. 4. pp. 247-255.
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