Seroprevalence and molecular characteristics of hepatitis E virus in household-raised pig population in the Philippines

Xiaofang Liu; Mariko Saito; Yusuke Sayama; Ellie Suzuki; Fedelino F. Malbas; Hazel O. Galang; Yuki Furuse; Mayuko Saito; Tiancheng Li; Akira Suzuki; Hitoshi Oshitani

doi:10.1186/s12917-015-0322-z

Seroprevalence and molecular characteristics of hepatitis E virus in household-raised pig population in the Philippines

Xiaofang Liu, Mariko Saito, Yusuke Sayama, Ellie Suzuki, Fedelino F. Malbas, Hazel O. Galang, Yuki Furuse, Mayuko Saito, Tiancheng Li, Akira Suzuki, Hitoshi Oshitani

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14 Scopus citations

Abstract

Background: Hepatitis E virus (HEV) infection is a significant public health concern in Asia, and swine is an important source of sporadic HEV infection in human. However, no epidemiological data are available regarding HEV infection among the swine or human population in the Philippines. To assess the HEV infection status among pigs in rural areas, we investigated the molecular characteristics and seroprevalence of HEV among household-raised pigs in San Jose, Tarlac Province, the Philippines. Result: Serum and rectal swab samples were collected from 299 pigs aged 2-24 months from 155 households in four barangays (villages) between July 2010 and June 2011. Enzyme-linked immunosorbent assay (ELISA) revealed that 50.3% [95% confidence interval (CI) 44.5-56.2%] and 22.9% (95% CI 18.2-28.1%) of pigs tested positive for anti-HEV IgG and IgM, respectively. HEV RNA was detected in the feces of 22 pigs (7.4%, 95% CI 4.7-10.9%). A total of 103 households (66.5%, 95% CI 58.4-73.8%) had at least one pig that tested positive for anti-HEV IgG or IgM or HEV RNA. The prevalence of anti-HEV IgG and IgM in breeding pig (8-24 months) were higher than that in growing pigs (2-4 months) (p

Original language	English (US)
Article number	11
Journal	BMC Veterinary Research
Volume	11
Issue number	1
DOIs	https://doi.org/10.1186/s12917-015-0322-z
State	Published - Jan 27 2015
Externally published	Yes

Keywords

Genotype 3
Hepatitis E virus
Household-raised pig
Philippines
Seroprevalence

ASJC Scopus subject areas

General Veterinary

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10.1186/s12917-015-0322-z

Cite this

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title = "Seroprevalence and molecular characteristics of hepatitis E virus in household-raised pig population in the Philippines",

abstract = "Background: Hepatitis E virus (HEV) infection is a significant public health concern in Asia, and swine is an important source of sporadic HEV infection in human. However, no epidemiological data are available regarding HEV infection among the swine or human population in the Philippines. To assess the HEV infection status among pigs in rural areas, we investigated the molecular characteristics and seroprevalence of HEV among household-raised pigs in San Jose, Tarlac Province, the Philippines. Result: Serum and rectal swab samples were collected from 299 pigs aged 2-24 months from 155 households in four barangays (villages) between July 2010 and June 2011. Enzyme-linked immunosorbent assay (ELISA) revealed that 50.3% [95% confidence interval (CI) 44.5-56.2%] and 22.9% (95% CI 18.2-28.1%) of pigs tested positive for anti-HEV IgG and IgM, respectively. HEV RNA was detected in the feces of 22 pigs (7.4%, 95% CI 4.7-10.9%). A total of 103 households (66.5%, 95% CI 58.4-73.8%) had at least one pig that tested positive for anti-HEV IgG or IgM or HEV RNA. The prevalence of anti-HEV IgG and IgM in breeding pig (8-24 months) were higher than that in growing pigs (2-4 months) (p",

keywords = "Genotype 3, Hepatitis E virus, Household-raised pig, Philippines, Seroprevalence",

author = "Xiaofang Liu and Mariko Saito and Yusuke Sayama and Ellie Suzuki and Malbas, {Fedelino F.} and Galang, {Hazel O.} and Yuki Furuse and Mayuko Saito and Tiancheng Li and Akira Suzuki and Hitoshi Oshitani",

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doi = "10.1186/s12917-015-0322-z",

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T1 - Seroprevalence and molecular characteristics of hepatitis E virus in household-raised pig population in the Philippines

AU - Liu, Xiaofang

AU - Saito, Mariko

AU - Sayama, Yusuke

AU - Suzuki, Ellie

AU - Malbas, Fedelino F.

AU - Galang, Hazel O.

AU - Furuse, Yuki

AU - Saito, Mayuko

AU - Li, Tiancheng

AU - Suzuki, Akira

AU - Oshitani, Hitoshi

PY - 2015/1/27

Y1 - 2015/1/27

N2 - Background: Hepatitis E virus (HEV) infection is a significant public health concern in Asia, and swine is an important source of sporadic HEV infection in human. However, no epidemiological data are available regarding HEV infection among the swine or human population in the Philippines. To assess the HEV infection status among pigs in rural areas, we investigated the molecular characteristics and seroprevalence of HEV among household-raised pigs in San Jose, Tarlac Province, the Philippines. Result: Serum and rectal swab samples were collected from 299 pigs aged 2-24 months from 155 households in four barangays (villages) between July 2010 and June 2011. Enzyme-linked immunosorbent assay (ELISA) revealed that 50.3% [95% confidence interval (CI) 44.5-56.2%] and 22.9% (95% CI 18.2-28.1%) of pigs tested positive for anti-HEV IgG and IgM, respectively. HEV RNA was detected in the feces of 22 pigs (7.4%, 95% CI 4.7-10.9%). A total of 103 households (66.5%, 95% CI 58.4-73.8%) had at least one pig that tested positive for anti-HEV IgG or IgM or HEV RNA. The prevalence of anti-HEV IgG and IgM in breeding pig (8-24 months) were higher than that in growing pigs (2-4 months) (p

AB - Background: Hepatitis E virus (HEV) infection is a significant public health concern in Asia, and swine is an important source of sporadic HEV infection in human. However, no epidemiological data are available regarding HEV infection among the swine or human population in the Philippines. To assess the HEV infection status among pigs in rural areas, we investigated the molecular characteristics and seroprevalence of HEV among household-raised pigs in San Jose, Tarlac Province, the Philippines. Result: Serum and rectal swab samples were collected from 299 pigs aged 2-24 months from 155 households in four barangays (villages) between July 2010 and June 2011. Enzyme-linked immunosorbent assay (ELISA) revealed that 50.3% [95% confidence interval (CI) 44.5-56.2%] and 22.9% (95% CI 18.2-28.1%) of pigs tested positive for anti-HEV IgG and IgM, respectively. HEV RNA was detected in the feces of 22 pigs (7.4%, 95% CI 4.7-10.9%). A total of 103 households (66.5%, 95% CI 58.4-73.8%) had at least one pig that tested positive for anti-HEV IgG or IgM or HEV RNA. The prevalence of anti-HEV IgG and IgM in breeding pig (8-24 months) were higher than that in growing pigs (2-4 months) (p

KW - Genotype 3

KW - Hepatitis E virus

KW - Household-raised pig

KW - Philippines

KW - Seroprevalence

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Seroprevalence and molecular characteristics of hepatitis E virus in household-raised pig population in the Philippines

Abstract

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