Serological response to plasmid-encoded antigens in children and adults with shigellosis

Peter Echeverria, Suvath Hanchalay, David N. Taylor

Research output: Contribution to journalArticle

Abstract

The immunological response to plasmid-encoded antigens of virulent Shigella was determined in Thai children less than 4 yr of age and in Thai adults by immunoblot analysis and ELISA. Forty-two percent ( 8 19) of Thai children and 4% ( 1 22) of Thai adults with shigellosis developed a ≥4-fold rise in IgG antibody titer to water-extracted antigens of Shigella flexneri M90T by ELISA (p = 0.006). Two children and one lactating mother with shigellosis developed a 4-fold rise in serum IgA antibody titers to water-extracted antigens of M90T. The results of the ELISA were confirmed by immunoblot analysis in all of the 41 paired sera examined. Five patients developed IgA, and four developed IgM, antibodies as detected by immunoblot analysis, that were not detected by ELISA. The reciprocal log2 geometric mean titers of antibodies to plasmid-encoded antigens in acute sera was higher in Thai adults than Thai children: IgG 7,265 versus 1,659; IgM 879 versus 480; and IgA 662 versus 60 (p < 0.001). Thai adults had high titers of antibodies to plasmid-encoded antigens in their acute sera, but were susceptible to Shigella infections, although they were historically less susceptible than Thai children.

Original languageEnglish (US)
Pages (from-to)75-80
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume10
Issue number2
DOIs
StatePublished - Jun 1988

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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