TY - JOUR
T1 - Serologic identification of multiple tumor-associated antigens on murine sarcomas
AU - Parker, George A.
AU - Rosenberg, Steven A.
N1 - Funding Information:
ABBREVIATIONS USED; MCA = 3-methylcholanthrene; HBSS = Hanks' balanced salt solution; CV = coefficient of variation; MCA-3HI = MCA-3 hyperimmune serum; MCA-3-28 = MCA-3 for 28 days. 1 Received May 6, 1976; accepted October 27, 1976. 2 Surgery Branch, National Cancer Institute, National Institutes of Health (NIH), Public Health Service, U.S. Department of Health, Education, and Welfare, Bethesda, Md. 20014.
PY - 1977/5
Y1 - 1977/5
N2 - The humoral immune response to two transplanted chemically induced murine sarcomas (MCA-2 and MCA-3) was studied in C57BL/6N mice. These tumors were immunogenic as evidenced by tumor amputation and rechallenge experiments, and no cross-reactivity between them was observed in in vivo challenge experiments. Utilizing a complement-dependent microcytotoxicity assay, we detected antibody to both MCA-2 and MCA-3 in the sera of animals bearing MCA-3 as well as after tumor removal. The sera of animals hyperimmunized to MCA-3 (MCA-3HI) was also cytotoxic in high titer to both MCA-2 and MCA-3 (50% cytotoxicity titers of 1:80 and 1:320, respectively). Sequential absorptions of sera from animals bearing MCA-3 and MCA-3HI sera with fresh MCA-2 cells completely removed activity against MCA-2 but retained reactivity to MCA-3. Sequential absorptions with fresh MCA-3 cells produced stepwise reductions of activity against both tumors, whereas absorption with normal cells produced no loss of activity against either tumor. Thus both specific and cross-reactive antigens were expressed on the surfaces of MCA-3 cells. Only the specific tumor antigen appeared to be involved in in vivo protection against tumor challenge.
AB - The humoral immune response to two transplanted chemically induced murine sarcomas (MCA-2 and MCA-3) was studied in C57BL/6N mice. These tumors were immunogenic as evidenced by tumor amputation and rechallenge experiments, and no cross-reactivity between them was observed in in vivo challenge experiments. Utilizing a complement-dependent microcytotoxicity assay, we detected antibody to both MCA-2 and MCA-3 in the sera of animals bearing MCA-3 as well as after tumor removal. The sera of animals hyperimmunized to MCA-3 (MCA-3HI) was also cytotoxic in high titer to both MCA-2 and MCA-3 (50% cytotoxicity titers of 1:80 and 1:320, respectively). Sequential absorptions of sera from animals bearing MCA-3 and MCA-3HI sera with fresh MCA-2 cells completely removed activity against MCA-2 but retained reactivity to MCA-3. Sequential absorptions with fresh MCA-3 cells produced stepwise reductions of activity against both tumors, whereas absorption with normal cells produced no loss of activity against either tumor. Thus both specific and cross-reactive antigens were expressed on the surfaces of MCA-3 cells. Only the specific tumor antigen appeared to be involved in in vivo protection against tumor challenge.
UR - http://www.scopus.com/inward/record.url?scp=0017687614&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017687614&partnerID=8YFLogxK
U2 - 10.1093/jnci/58.5.1303
DO - 10.1093/jnci/58.5.1303
M3 - Article
C2 - 67211
AN - SCOPUS:0017687614
SN - 0027-8874
VL - 58
SP - 1303
EP - 1309
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 5
ER -