Serodiagnosis of Rocky Mountain spotted fever: Comparison of IgM and IgG enzyme-linked immunosorbent assays and indirect fluorescent antibody test

M. L. Clements, J. S. Dumler, P. Fiset, C. L. Wisseman, M. J. Snyder, M. M. Levine

Research output: Contribution to journalArticle

Abstract

To characterize IgM and IgG antibody responses in Rocky Mountain spotted fever (RMSF), a microtiter enzyme-linked immunosorbent assay (ELISA) using density gradient-purified Rickettsia rickettsii as antigen was developed. Sera of vaccinated individuals and patients with RMSF were tested by ELISA and by indirect fluorescent antibody (IFA) tests. Diagnostic agreement between ELISA and the IFA test was 76% and 52% for IgG and IgM antibody, respectively. Diagnostic agreement between the ELISA for IgG antibody and the IFA test for total immunoglobulins was 84%. The ELISAs for IgM and IgG antibody were as specific (100%) and as sensitive (100%) as the IFA test (83%-100%) in detecting antibody increases in paired sera from persons with RMSF and were superior to the IFA test in detecting seroconversions in vaccines. The ELISA also detected antibodies in a single convalescent-phase serum with sensitivity and reliability. The ELISA for IgG antibody is appropriate for seroepidemiology and serodiagnosis sine it permits measurement of antibody at a single dilution of serum up to a year after illness.

Original languageEnglish (US)
Pages (from-to)876-880
Number of pages5
JournalJournal of Infectious Diseases
Volume148
Issue number5
DOIs
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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