Serodiagnosis of Rocky Mountain spotted fever: Comparison of IgM and IgG enzyme-linked immunosorbent assays and indirect fluorescent antibody test

M. L. Clements; J. S. Dumler; P. Fiset; C. L. Wisseman; M. J. Snyder; M. M. Levine

doi:10.1093/infdis/148.5.876

Serodiagnosis of Rocky Mountain spotted fever: Comparison of IgM and IgG enzyme-linked immunosorbent assays and indirect fluorescent antibody test

M. L. Clements, J. S. Dumler, P. Fiset, C. L. Wisseman, M. J. Snyder, M. M. Levine

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

To characterize IgM and IgG antibody responses in Rocky Mountain spotted fever (RMSF), a microtiter enzyme-linked immunosorbent assay (ELISA) using density gradient-purified Rickettsia rickettsii as antigen was developed. Sera of vaccinated individuals and patients with RMSF were tested by ELISA and by indirect fluorescent antibody (IFA) tests. Diagnostic agreement between ELISA and the IFA test was 76% and 52% for IgG and IgM antibody, respectively. Diagnostic agreement between the ELISA for IgG antibody and the IFA test for total immunoglobulins was 84%. The ELISAs for IgM and IgG antibody were as specific (100%) and as sensitive (100%) as the IFA test (83%-100%) in detecting antibody increases in paired sera from persons with RMSF and were superior to the IFA test in detecting seroconversions in vaccines. The ELISA also detected antibodies in a single convalescent-phase serum with sensitivity and reliability. The ELISA for IgG antibody is appropriate for seroepidemiology and serodiagnosis sine it permits measurement of antibody at a single dilution of serum up to a year after illness.

Original language	English (US)
Pages (from-to)	876-880
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	148
Issue number	5
DOIs	https://doi.org/10.1093/infdis/148.5.876
State	Published - 1983
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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10.1093/infdis/148.5.876

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@article{9295193687444c84bdaf7e315a1a0629,

title = "Serodiagnosis of Rocky Mountain spotted fever: Comparison of IgM and IgG enzyme-linked immunosorbent assays and indirect fluorescent antibody test",

abstract = "To characterize IgM and IgG antibody responses in Rocky Mountain spotted fever (RMSF), a microtiter enzyme-linked immunosorbent assay (ELISA) using density gradient-purified Rickettsia rickettsii as antigen was developed. Sera of vaccinated individuals and patients with RMSF were tested by ELISA and by indirect fluorescent antibody (IFA) tests. Diagnostic agreement between ELISA and the IFA test was 76% and 52% for IgG and IgM antibody, respectively. Diagnostic agreement between the ELISA for IgG antibody and the IFA test for total immunoglobulins was 84%. The ELISAs for IgM and IgG antibody were as specific (100%) and as sensitive (100%) as the IFA test (83%-100%) in detecting antibody increases in paired sera from persons with RMSF and were superior to the IFA test in detecting seroconversions in vaccines. The ELISA also detected antibodies in a single convalescent-phase serum with sensitivity and reliability. The ELISA for IgG antibody is appropriate for seroepidemiology and serodiagnosis sine it permits measurement of antibody at a single dilution of serum up to a year after illness.",

author = "Clements, {M. L.} and Dumler, {J. S.} and P. Fiset and Wisseman, {C. L.} and Snyder, {M. J.} and Levine, {M. M.}",

note = "Funding Information: Received for publication April 29, 1983, and in revised form June 23, 1983. This work was presented in part at the 20th Interscience Conference on Antimicrobial Agents and Chemotherapy, held in Miami, Florida, in October 1982. Written, informed consent was obtained from the volunteers, and the study protocols were approved by the Human Volunteers Research Committee of the University of Maryland and by the Clinical Review Subpanel of the National Institute of Allergy and Infectious Diseases. This work was supported in part by contract NOIAI 12666 from the National Institute of Allergy and Infectious Diseases, and computer support was provided by the Computer Science Center of the University of Maryland. Please address requests for reprints to Dr M. L. Clements, Center for Vaccine Development, University of Maryland School of Medicine, 29 South Greene Street, Baltimore, Maryland 21201.",

year = "1983",

doi = "10.1093/infdis/148.5.876",

language = "English (US)",

volume = "148",

pages = "876--880",

journal = "Journal of Infectious Diseases",

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T1 - Serodiagnosis of Rocky Mountain spotted fever

T2 - Comparison of IgM and IgG enzyme-linked immunosorbent assays and indirect fluorescent antibody test

AU - Clements, M. L.

AU - Dumler, J. S.

AU - Fiset, P.

AU - Wisseman, C. L.

AU - Snyder, M. J.

AU - Levine, M. M.

N1 - Funding Information: Received for publication April 29, 1983, and in revised form June 23, 1983. This work was presented in part at the 20th Interscience Conference on Antimicrobial Agents and Chemotherapy, held in Miami, Florida, in October 1982. Written, informed consent was obtained from the volunteers, and the study protocols were approved by the Human Volunteers Research Committee of the University of Maryland and by the Clinical Review Subpanel of the National Institute of Allergy and Infectious Diseases. This work was supported in part by contract NOIAI 12666 from the National Institute of Allergy and Infectious Diseases, and computer support was provided by the Computer Science Center of the University of Maryland. Please address requests for reprints to Dr M. L. Clements, Center for Vaccine Development, University of Maryland School of Medicine, 29 South Greene Street, Baltimore, Maryland 21201.

PY - 1983

Y1 - 1983

N2 - To characterize IgM and IgG antibody responses in Rocky Mountain spotted fever (RMSF), a microtiter enzyme-linked immunosorbent assay (ELISA) using density gradient-purified Rickettsia rickettsii as antigen was developed. Sera of vaccinated individuals and patients with RMSF were tested by ELISA and by indirect fluorescent antibody (IFA) tests. Diagnostic agreement between ELISA and the IFA test was 76% and 52% for IgG and IgM antibody, respectively. Diagnostic agreement between the ELISA for IgG antibody and the IFA test for total immunoglobulins was 84%. The ELISAs for IgM and IgG antibody were as specific (100%) and as sensitive (100%) as the IFA test (83%-100%) in detecting antibody increases in paired sera from persons with RMSF and were superior to the IFA test in detecting seroconversions in vaccines. The ELISA also detected antibodies in a single convalescent-phase serum with sensitivity and reliability. The ELISA for IgG antibody is appropriate for seroepidemiology and serodiagnosis sine it permits measurement of antibody at a single dilution of serum up to a year after illness.

AB - To characterize IgM and IgG antibody responses in Rocky Mountain spotted fever (RMSF), a microtiter enzyme-linked immunosorbent assay (ELISA) using density gradient-purified Rickettsia rickettsii as antigen was developed. Sera of vaccinated individuals and patients with RMSF were tested by ELISA and by indirect fluorescent antibody (IFA) tests. Diagnostic agreement between ELISA and the IFA test was 76% and 52% for IgG and IgM antibody, respectively. Diagnostic agreement between the ELISA for IgG antibody and the IFA test for total immunoglobulins was 84%. The ELISAs for IgM and IgG antibody were as specific (100%) and as sensitive (100%) as the IFA test (83%-100%) in detecting antibody increases in paired sera from persons with RMSF and were superior to the IFA test in detecting seroconversions in vaccines. The ELISA also detected antibodies in a single convalescent-phase serum with sensitivity and reliability. The ELISA for IgG antibody is appropriate for seroepidemiology and serodiagnosis sine it permits measurement of antibody at a single dilution of serum up to a year after illness.

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Serodiagnosis of Rocky Mountain spotted fever: Comparison of IgM and IgG enzyme-linked immunosorbent assays and indirect fluorescent antibody test

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