Serial analysis of gene expression

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Abstract

The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.

Original languageEnglish (US)
Pages (from-to)484-487
Number of pages4
JournalScience
Volume270
Issue number5235
DOIs
StatePublished - Jan 1 1995

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ASJC Scopus subject areas

  • General

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