Sequential gene promoter interactions of C/EBPβ, C/EBPα, and PPARγ during adipogenesis

Qi Qun Tang, Jiang Wen Zhang, M. Daniel Lane

Research output: Contribution to journalArticle

Abstract

Treatment of 3T3-L1 preadipocytes with differentiation inducers triggers a cascade in which C/EBPβ is rapidly expressed, followed by C/EBPα and PPARγ. C/EBPα and PPARγ then activate the expression of adipocyte genes that produce the differentiated phenotype. Circumstantial evidence indicates that C/EBPβ activates transcription of the C/EBPα and PPARγ genes, both of which possess C/EBP regulatory elements in their proximal promoters. Although C/EBPβ is expressed immediately upon induction of differentiation, acquisition of DNA binding activity is delayed for ∼14h. Chromatin immunoprecipitation (ChIP) analysis conducted 24h after induction revealed that C/EBPβ binds to C/EBP regulatory elements in the proximal promoters of the C/EBPα and PPARγ genes. After an additional delay ChIP analysis showed that C/EBPα binds to its own promoter and to the promoters of the PPARγ and 422/aP2 genes. These findings support the view that once expressed, C/EBPα is responsible for maintaining the expression of PPARγ and C/EBPα, as well as adipocyte proteins (e.g., 422/aP2) in the terminally differentiated state. Together these findings provide compelling evidence that C/EBPβ, C/EBPα, and PPARγ participate in a cascade during adipogenesis.

Original languageEnglish (US)
Pages (from-to)235-239
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume319
Issue number1
DOIs
StatePublished - Jun 18 2004

Fingerprint

Adipogenesis
Peroxisome Proliferator-Activated Receptors
Genes
Chromatin Immunoprecipitation
Adipocytes
Chromatin
Transcription
Phenotype
Gene Expression
DNA

Keywords

  • 3T3-L1
  • Adipocyte differentiation
  • C/EBPα
  • C/EBPβ
  • ChIP
  • PPARγ

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sequential gene promoter interactions of C/EBPβ, C/EBPα, and PPARγ during adipogenesis. / Tang, Qi Qun; Zhang, Jiang Wen; Lane, M. Daniel.

In: Biochemical and Biophysical Research Communications, Vol. 319, No. 1, 18.06.2004, p. 235-239.

Research output: Contribution to journalArticle

Tang, Qi Qun ; Zhang, Jiang Wen ; Lane, M. Daniel. / Sequential gene promoter interactions of C/EBPβ, C/EBPα, and PPARγ during adipogenesis. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 319, No. 1. pp. 235-239.
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