Sequencing of Candidate Chromosome Instability Genes in Endometrial Cancers Reveals Somatic Mutations in ESCO1, CHTF18, and MRE11A

Jessica C. Price, Lana M. Pollock, Meghan L. Rudd, Sarah K. Fogoros, Hassan Mohamed, Christin L. Hanigan, Matthieu Le Gallo, Suiyuan Zhang, Pedro Cruz, Praveen F. Cherukuri, Nancy F. Hansen, Kirk J. McManus, Andrew K. Godwin, Dennis C. Sgroi, James C. Mullikin, Maria J. Merino, Philip Hieter, Daphne W. Bell

Research output: Contribution to journalArticle

Abstract

Most endometrial cancers can be classified histologically as endometrioid, serous, or clear cell. Non-endometrioid endometrial cancers (NEECs; serous and clear cell) are the most clinically aggressive of the three major histotypes and are characterized by aneuploidy, a feature of chromosome instability. The genetic alterations that underlie chromosome instability in endometrial cancer are poorly understood. In the present study, we used Sanger sequencing to search for nucleotide variants in the coding exons and splice junctions of 21 candidate chromosome instability genes, including 19 genes implicated in sister chromatid cohesion, from 24 primary, microsatellite-stable NEECs. Somatic mutations were verified by sequencing matched normal DNAs. We subsequently resequenced mutated genes from 41 additional NEECs as well as 42 endometrioid ECs (EECs). We uncovered nonsynonymous somatic mutations in ESCO1, CHTF18, and MRE11A in, respectively, 3.7% (4 of 107), 1.9% (2 of 107), and 1.9% (2 of 107) of endometrial tumors. Overall, 7.7% (5 of 65) of NEECs and 2.4% (1 of 42) of EECs had somatically mutated one or more of the three genes. A subset of mutations are predicted to impact protein function. The co-occurrence of somatic mutations in ESCO1 and CHTF18 was statistically significant (P = 0.0011, two-tailed Fisher's exact test). This is the first report of somatic mutations within ESCO1 and CHTF18 in endometrial tumors and of MRE11A mutations in microsatellite-stable endometrial tumors. Our findings warrant future studies to determine whether these mutations are driver events that contribute to the pathogenesis of endometrial cancer.

Original languageEnglish (US)
Article numbere63313
JournalPLoS One
Volume8
Issue number6
DOIs
StatePublished - Jun 3 2013
Externally publishedYes

Fingerprint

somatic mutation
Chromosomal Instability
Endometrial Neoplasms
Chromosomes
Genes
chromosomes
Tumors
Mutation
neoplasms
Microsatellite Repeats
genes
mutation
Set theory
Exons
microsatellite repeats
Nucleotides
Neoplasms
Chromatids
chromatids
aneuploidy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Price, J. C., Pollock, L. M., Rudd, M. L., Fogoros, S. K., Mohamed, H., Hanigan, C. L., ... Bell, D. W. (2013). Sequencing of Candidate Chromosome Instability Genes in Endometrial Cancers Reveals Somatic Mutations in ESCO1, CHTF18, and MRE11A. PLoS One, 8(6), [e63313]. https://doi.org/10.1371/journal.pone.0063313

Sequencing of Candidate Chromosome Instability Genes in Endometrial Cancers Reveals Somatic Mutations in ESCO1, CHTF18, and MRE11A. / Price, Jessica C.; Pollock, Lana M.; Rudd, Meghan L.; Fogoros, Sarah K.; Mohamed, Hassan; Hanigan, Christin L.; Le Gallo, Matthieu; Zhang, Suiyuan; Cruz, Pedro; Cherukuri, Praveen F.; Hansen, Nancy F.; McManus, Kirk J.; Godwin, Andrew K.; Sgroi, Dennis C.; Mullikin, James C.; Merino, Maria J.; Hieter, Philip; Bell, Daphne W.

In: PLoS One, Vol. 8, No. 6, e63313, 03.06.2013.

Research output: Contribution to journalArticle

Price, JC, Pollock, LM, Rudd, ML, Fogoros, SK, Mohamed, H, Hanigan, CL, Le Gallo, M, Zhang, S, Cruz, P, Cherukuri, PF, Hansen, NF, McManus, KJ, Godwin, AK, Sgroi, DC, Mullikin, JC, Merino, MJ, Hieter, P & Bell, DW 2013, 'Sequencing of Candidate Chromosome Instability Genes in Endometrial Cancers Reveals Somatic Mutations in ESCO1, CHTF18, and MRE11A', PLoS One, vol. 8, no. 6, e63313. https://doi.org/10.1371/journal.pone.0063313
Price, Jessica C. ; Pollock, Lana M. ; Rudd, Meghan L. ; Fogoros, Sarah K. ; Mohamed, Hassan ; Hanigan, Christin L. ; Le Gallo, Matthieu ; Zhang, Suiyuan ; Cruz, Pedro ; Cherukuri, Praveen F. ; Hansen, Nancy F. ; McManus, Kirk J. ; Godwin, Andrew K. ; Sgroi, Dennis C. ; Mullikin, James C. ; Merino, Maria J. ; Hieter, Philip ; Bell, Daphne W. / Sequencing of Candidate Chromosome Instability Genes in Endometrial Cancers Reveals Somatic Mutations in ESCO1, CHTF18, and MRE11A. In: PLoS One. 2013 ; Vol. 8, No. 6.
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abstract = "Most endometrial cancers can be classified histologically as endometrioid, serous, or clear cell. Non-endometrioid endometrial cancers (NEECs; serous and clear cell) are the most clinically aggressive of the three major histotypes and are characterized by aneuploidy, a feature of chromosome instability. The genetic alterations that underlie chromosome instability in endometrial cancer are poorly understood. In the present study, we used Sanger sequencing to search for nucleotide variants in the coding exons and splice junctions of 21 candidate chromosome instability genes, including 19 genes implicated in sister chromatid cohesion, from 24 primary, microsatellite-stable NEECs. Somatic mutations were verified by sequencing matched normal DNAs. We subsequently resequenced mutated genes from 41 additional NEECs as well as 42 endometrioid ECs (EECs). We uncovered nonsynonymous somatic mutations in ESCO1, CHTF18, and MRE11A in, respectively, 3.7{\%} (4 of 107), 1.9{\%} (2 of 107), and 1.9{\%} (2 of 107) of endometrial tumors. Overall, 7.7{\%} (5 of 65) of NEECs and 2.4{\%} (1 of 42) of EECs had somatically mutated one or more of the three genes. A subset of mutations are predicted to impact protein function. The co-occurrence of somatic mutations in ESCO1 and CHTF18 was statistically significant (P = 0.0011, two-tailed Fisher's exact test). This is the first report of somatic mutations within ESCO1 and CHTF18 in endometrial tumors and of MRE11A mutations in microsatellite-stable endometrial tumors. Our findings warrant future studies to determine whether these mutations are driver events that contribute to the pathogenesis of endometrial cancer.",
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