Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter

R. Hen, P. Sassone-Corsi, Jeffry Lynn Corden, M. P. Gaub, P. Chambon

Research output: Contribution to journalArticle

Abstract

We show that sequences located upstream from the T-A-T-A box, between positions -97 and -34, are necessary for efficient in vivo transcription from the adenovirus serotype 2 major late promoter. The effect of these upstream sequences was also investigated in vitro using a whole cell or an S100 extract and circular or linear templates. With the whole cell extract, the in vivo effect of the upstream sequences was reproduced in vitro. With the S100 extract, some effect of the upstream sequences was observed with circular, but not with linear, templates.

Original languageEnglish (US)
Pages (from-to)7132-7136
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number23 I
DOIs
StatePublished - 1982
Externally publishedYes

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Adenoviridae
Cell Extracts
In Vitro Techniques
Serogroup

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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abstract = "We show that sequences located upstream from the T-A-T-A box, between positions -97 and -34, are necessary for efficient in vivo transcription from the adenovirus serotype 2 major late promoter. The effect of these upstream sequences was also investigated in vitro using a whole cell or an S100 extract and circular or linear templates. With the whole cell extract, the in vivo effect of the upstream sequences was reproduced in vitro. With the S100 extract, some effect of the upstream sequences was observed with circular, but not with linear, templates.",
author = "R. Hen and P. Sassone-Corsi and Corden, {Jeffry Lynn} and Gaub, {M. P.} and P. Chambon",
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language = "English (US)",
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journal = "Proceedings of the National Academy of Sciences of the United States of America",
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T1 - Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter

AU - Hen, R.

AU - Sassone-Corsi, P.

AU - Corden, Jeffry Lynn

AU - Gaub, M. P.

AU - Chambon, P.

PY - 1982

Y1 - 1982

N2 - We show that sequences located upstream from the T-A-T-A box, between positions -97 and -34, are necessary for efficient in vivo transcription from the adenovirus serotype 2 major late promoter. The effect of these upstream sequences was also investigated in vitro using a whole cell or an S100 extract and circular or linear templates. With the whole cell extract, the in vivo effect of the upstream sequences was reproduced in vitro. With the S100 extract, some effect of the upstream sequences was observed with circular, but not with linear, templates.

AB - We show that sequences located upstream from the T-A-T-A box, between positions -97 and -34, are necessary for efficient in vivo transcription from the adenovirus serotype 2 major late promoter. The effect of these upstream sequences was also investigated in vitro using a whole cell or an S100 extract and circular or linear templates. With the whole cell extract, the in vivo effect of the upstream sequences was reproduced in vitro. With the S100 extract, some effect of the upstream sequences was observed with circular, but not with linear, templates.

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U2 - 10.1073/pnas.79.23.7132

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 23 I

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