Sequence comparisons of the anemia- and polycythemia-inducing strains of friend spleen focus-forming virus

L. Wolff, J. Kaminchik, W. D. Hankins, S. K. Ruscetti

Research output: Contribution to journalArticle

Abstract

A nucleotide sequence analysis carried out on the envelope gene of the anemia-inducing strain of the Friend spleen focus-forming virus (F-SFFV(A)) reveals that its product has some unique features in common with previously described polycythemia-inducing strains of F-SFFV (F-SFFV(P)). (i) It contains an amino terminus that is highly related to the gp70 of mink cell focus-inducing viruses, (ii) it is a fusion protein containing the amino terminus of gp70 and the carboxy terminus of p15E, and (iii) it lacks the R-peptide normally found at the carboxy end of the p15E region. Although the envelope genes of F-SFFV(A) and F-SFFV(P) are quite similar overall, they do show sequence variation, particularly at the 3' end in the p15E-related region. These variations may contribute to previously observed differences in the response of F-SFFV(P)- and F-SFFV(A)-infected erythroid cells to regulatory hormone or to differences in the way the envelope glycoproteins are processed. The long terminal repeat regions of F-SFFV(A) and the Lilly-Steeves strain of F-SFFV(P) were also sequenced and compared with each other and with a previously published sequence of another F-SFFV(P) long terminal repeat. The sequences were found to be reasonably similar to each other but different from their ecotropic parent, Friend murine leukemia virus, as a result of a deletion of one copy of the direct tandem repeat in the enhancer regions. The observation that all SFFVs have this common change in the long terminal repeat enhancer region raises the possibility that it is required for pathogenicity.

Original languageEnglish (US)
Pages (from-to)570-578
Number of pages9
JournalJournal of Virology
Volume53
Issue number2
StatePublished - 1985
Externally publishedYes

Fingerprint

Spleen Focus-Forming Viruses
polycythemia
Polycythemia
terminal repeat sequences
anemia
Anemia
spleen
viruses
Murine leukemia virus
tandem repeat sequences
mink
Terminal Repeat Sequences
glycoproteins
pathogenicity
genes
sequence analysis
hormones
peptides
cells
nucleotide sequences

ASJC Scopus subject areas

  • Immunology

Cite this

Wolff, L., Kaminchik, J., Hankins, W. D., & Ruscetti, S. K. (1985). Sequence comparisons of the anemia- and polycythemia-inducing strains of friend spleen focus-forming virus. Journal of Virology, 53(2), 570-578.

Sequence comparisons of the anemia- and polycythemia-inducing strains of friend spleen focus-forming virus. / Wolff, L.; Kaminchik, J.; Hankins, W. D.; Ruscetti, S. K.

In: Journal of Virology, Vol. 53, No. 2, 1985, p. 570-578.

Research output: Contribution to journalArticle

Wolff, L, Kaminchik, J, Hankins, WD & Ruscetti, SK 1985, 'Sequence comparisons of the anemia- and polycythemia-inducing strains of friend spleen focus-forming virus', Journal of Virology, vol. 53, no. 2, pp. 570-578.
Wolff, L. ; Kaminchik, J. ; Hankins, W. D. ; Ruscetti, S. K. / Sequence comparisons of the anemia- and polycythemia-inducing strains of friend spleen focus-forming virus. In: Journal of Virology. 1985 ; Vol. 53, No. 2. pp. 570-578.
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