Sequence and expression of testis-expressed gene 14 (Tex14): A gene encoding a protein kinase preferentially expressed during spermatogenesis

Meng Hsing Wu, Aleksandar Rajkovic, Kathleen H. Burns, Wei Yan, Yi Nan Lin, Martin M. Matzuk

Research output: Contribution to journalArticle

Abstract

To discover germ cell-specific genes, we used in silico subtraction and identified testis expressed gene 14 (Tex14). Mouse Tex14 contains an open reading frame encoding a 1450-amino-acid protein, which shares 64% amino acid identity with the predicted human TEX14 protein. The predicted TEX14 amino acid sequence consists of three ankyrin repeats, a protein kinase domain, and a leucine zipper dimerization motif. Northern blot analysis and in situ hybridization show that Tex14 mRNA is expressed specifically in the testis, with highest levels observed in pachytene, diplotene, and meiotically dividing spermatocytes. Two 5′ splice variants of mouse Tex14 were discovered by sequencing 5′-RACE polymerase chain reaction products. TEX14 is predicted to be localized to the nucleus, suggesting that it may play a key role in regulating gene expression or modulating nuclear events during mammalian spermatogenesis.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalGene Expression Patterns
Volume3
Issue number2
DOIs
StatePublished - May 1 2003
Externally publishedYes

Keywords

  • Ankyrin repeats
  • Germ cell, In silico subtraction
  • Rapid amplification of cDNA ends

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Developmental Biology

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