Sequence analysis of closely related retrotransposon families from fission yeast

Daniel C. Weaver, George V. Shpakovski, Emerita Caputo, Henry L. Levin, J. D. Bocke

Research output: Contribution to journalArticle

Abstract

Two families of retrotransposons, Tf1 and Tf2, have been isolated from the fission yeast, Schizosaccharomyces pombe. We report here the nucleotide (nt) sequence of a Tf2 element, the only retrotransposon family known from the commonly used laboratory strains, 972 and 975, and their derivatives. The total nt sequence of Tf2 was derived from the complete sequence of the coding region and 3′ long terminal repeat (LTR) of randomly cloned element Tf2-1, and from a full 5′ LTR and approximately one-third of the open reading frame (ORF) of Tf2-43, a Tf2 element found in the head-to-head orientation adjacent to the Sz. pombe rpb6 gene. The two Tf2 sequences are nearly identical and both of them contain a single ORF encoding a protein with regions of sequence similar to protease, reverse transcriptase, RNase H (RH) and integrase from other retrotransposons and retroviruses. Sequence comparisons between Tf1 and Tf 2 indicate an extreme divergence of the putative capsid protein-encoding regions of these two elements, as well as divergence of a segment of the LTR, but otherwise virtually identical sequence.

Original languageEnglish (US)
Pages (from-to)135-139
Number of pages5
JournalGene
Volume131
Issue number1
DOIs
StatePublished - Sep 6 1993
Externally publishedYes

Fingerprint

Retroelements
Terminal Repeat Sequences
Schizosaccharomyces
Sequence Analysis
Open Reading Frames
Ribonuclease H
Integrases
RNA-Directed DNA Polymerase
Capsid Proteins
Retroviridae
Peptide Hydrolases
Genes
Proteins

Keywords

  • complete nucleotide sequence
  • evolution
  • rpb6 gene
  • Schizosaccharomyces pombe
  • Tf2 element
  • transposable elements

ASJC Scopus subject areas

  • Genetics

Cite this

Weaver, D. C., Shpakovski, G. V., Caputo, E., Levin, H. L., & Bocke, J. D. (1993). Sequence analysis of closely related retrotransposon families from fission yeast. Gene, 131(1), 135-139. https://doi.org/10.1016/0378-1119(93)90682-S

Sequence analysis of closely related retrotransposon families from fission yeast. / Weaver, Daniel C.; Shpakovski, George V.; Caputo, Emerita; Levin, Henry L.; Bocke, J. D.

In: Gene, Vol. 131, No. 1, 06.09.1993, p. 135-139.

Research output: Contribution to journalArticle

Weaver, DC, Shpakovski, GV, Caputo, E, Levin, HL & Bocke, JD 1993, 'Sequence analysis of closely related retrotransposon families from fission yeast', Gene, vol. 131, no. 1, pp. 135-139. https://doi.org/10.1016/0378-1119(93)90682-S
Weaver DC, Shpakovski GV, Caputo E, Levin HL, Bocke JD. Sequence analysis of closely related retrotransposon families from fission yeast. Gene. 1993 Sep 6;131(1):135-139. https://doi.org/10.1016/0378-1119(93)90682-S
Weaver, Daniel C. ; Shpakovski, George V. ; Caputo, Emerita ; Levin, Henry L. ; Bocke, J. D. / Sequence analysis of closely related retrotransposon families from fission yeast. In: Gene. 1993 ; Vol. 131, No. 1. pp. 135-139.
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