Separation, purification and identification of human apolipoprotein H

Wang Cong, Sun Yi-Hong, Wang Xue-Fei, Ma Duan, Li Xi, Tang Qi-Qun, Qin Xin-Yu

Research output: Contribution to journalArticlepeer-review

Abstract

Objective To explore a method to separate and purify conveniently and efficiently apolipoprotein H from human plasma. Methods We added perchloric acid into human plasma. Apolipoprotein H was separated and purified by ion exchange chromatography and affinity chromatography. The purified apolipoprotein H was analyzed by SDS-polyacrylamide gel electrophoresis, Western blot and BCA kit. Results We repeated the experiment three times. The purity of the apolipoprotein H was all over 98%. Western blot demonstrated that anti β2- Glycoprotein I monoclonal antibody reacted with the purified apolipoprotein H. We obtained a total of 12. 56 mg purified apolipoprotein H, average 21 mg/L. Conclusions This method has good repeatability, reliability and more convenient and economical than the traditional method.

Original languageEnglish (US)
Pages (from-to)61-64
Number of pages4
JournalFudan University Journal of Medical Sciences
Volume36
Issue number1
StatePublished - Jan 2009
Externally publishedYes

Keywords

  • Apolipoprotein H
  • Identify
  • Purify
  • Separate

ASJC Scopus subject areas

  • Medicine(all)

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