Separation of rat bone marrow cells by counterflow centrifugal elutriation: a model for studying the effects of lymphocyte depletion

J. E. Wagner, A. D. Donnenberg, S. J. Noga, J. M. Wiley, A. M. Yeager, G. B. Vogelsang, A. D. Hess, G. W. Santos

Research output: Contribution to journalArticle

Abstract

We have developed a simple three flow-rate, fixed rotor speed, counterflow centrifugal elutriation (CCE) procedure that permits the isolation of an engraftable lymphocyte-depleted (>98%) fraction from ACI rat bone marrow. The different cell fractions were characterized by morphology, alloreactivity in mixed lymphocyte culture and limiting dilution analysis, colony-forming capacity, and their capacity to reconstitute hematopoiesis and effect a graft-versus-host reaction in lethally irradiated allogeneic hosts. After CCE fractionation of ACI rat marrow, transplantation of the lymphocyte-depleted marrow fraction resulted in sustained engraftment without evidence of clinical or histologic acute graft-versus-host disease (GVHD). CCE fractionation of rat bone marrow may be useful preclinical model for studying lympho-hematopoietic and immune reconstitution after transplantation with lymphocyte-depleted donor marrow, as well as for studying the role of lymphocyte subpopulations on engraftment, acute GVHD, and leukemia relapse in syngeneic and allogeneic bone marrow transplantation.

Original languageEnglish (US)
Pages (from-to)206-212
Number of pages7
JournalExperimental Hematology
Volume16
Issue number3
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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    Wagner, J. E., Donnenberg, A. D., Noga, S. J., Wiley, J. M., Yeager, A. M., Vogelsang, G. B., Hess, A. D., & Santos, G. W. (1988). Separation of rat bone marrow cells by counterflow centrifugal elutriation: a model for studying the effects of lymphocyte depletion. Experimental Hematology, 16(3), 206-212.