Separation of mannosylretinylphosphate from dolichylmannosylphosphate by chromatography on columns of DEAE-Sephacel

Wlodzimierz Sasak; Carol S. Silverman-Jones; Luigi M. De Luca

doi:10.1016/0003-2697(79)90075-7

Separation of mannosylretinylphosphate from dolichylmannosylphosphate by chromatography on columns of DEAE-Sephacel

Wlodzimierz Sasak, Carol S. Silverman-Jones, Luigi M. De Luca

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A one-step column chromatographic procedure on DEAE-Sephacel allows the separation of mannosylretinylphosphate from dolichylmannosylphosphate with minimal breakdown of the mannosylretinylphosphate. Using this procedure, subcellular fractions of rat liver were shown to be active in synthesizing both mannolipids from GDP-[¹⁴C]mannose in the absence or presence of exogenous retinylphosphate.

Original language	English (US)
Pages (from-to)	298-301
Number of pages	4
Journal	Analytical biochemistry
Volume	97
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(79)90075-7
State	Published - Sep 1 1979
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "A one-step column chromatographic procedure on DEAE-Sephacel allows the separation of mannosylretinylphosphate from dolichylmannosylphosphate with minimal breakdown of the mannosylretinylphosphate. Using this procedure, subcellular fractions of rat liver were shown to be active in synthesizing both mannolipids from GDP-[14C]mannose in the absence or presence of exogenous retinylphosphate.",

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AU - De Luca, Luigi M.

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AB - A one-step column chromatographic procedure on DEAE-Sephacel allows the separation of mannosylretinylphosphate from dolichylmannosylphosphate with minimal breakdown of the mannosylretinylphosphate. Using this procedure, subcellular fractions of rat liver were shown to be active in synthesizing both mannolipids from GDP-[14C]mannose in the absence or presence of exogenous retinylphosphate.

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Separation of mannosylretinylphosphate from dolichylmannosylphosphate by chromatography on columns of DEAE-Sephacel

Abstract

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