Separation of mannosylretinylphosphate from dolichylmannosylphosphate by chromatography on columns of DEAE-Sephacel

Wlodzimierz Sasak, Carol S. Silverman-Jones, Luigi M. De Luca

Research output: Contribution to journalArticle

Abstract

A one-step column chromatographic procedure on DEAE-Sephacel allows the separation of mannosylretinylphosphate from dolichylmannosylphosphate with minimal breakdown of the mannosylretinylphosphate. Using this procedure, subcellular fractions of rat liver were shown to be active in synthesizing both mannolipids from GDP-[14C]mannose in the absence or presence of exogenous retinylphosphate.

Original languageEnglish (US)
Pages (from-to)298-301
Number of pages4
JournalAnalytical biochemistry
Volume97
Issue number2
DOIs
StatePublished - Sep 1 1979
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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