Separation of major prostaglandins, leukotrienes, and monoHETEs by high performance liquid chromatography

Stephen P. Peters, Edward S. Schulman, Mark C. Liu, Edward C. Hayes, Lawrence M. Lichtenstein

Research output: Contribution to journalArticlepeer-review

Abstract

A procedure using high performance liquid chromatography (HPLC) is described for the separation of major primary cyclooxygenase metabolites (prostacyclin metabolite-6ketoPGF, thromboxane B2, and prostaglandins F, E2, and D2), leukotrienes (C4, B4, and D4), monohydroxyeicosatetraenoic acids (15-, 11-, 12-, and 5HETEs), and free arachidonic acid. It is therefore possible to quantitate major arachidonic acid metabolites by a single chromatographic procedure. Using this technique we have determined that a major arachidonic acid metabolite of human lung macrophages co-elutes with leukotriene B4.

Original languageEnglish (US)
Pages (from-to)335-343
Number of pages9
JournalJournal of Immunological Methods
Volume64
Issue number3
DOIs
StatePublished - Nov 25 1983

Keywords

  • HETEs
  • HPLC
  • arachidonic acid
  • human lung macrophages
  • leukotrienes
  • prostaglandins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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