Separation of α- and β-globin messenger RNAs by formamide gel electrophoresis

Haig H. Kazazian, Pamela G. Snyder, Cheng Tu-chen

Research output: Contribution to journalArticlepeer-review

Abstract

Electrophoresis on 7.5% polyacrylamide gels in 98% formamide splits the 10s RNA of EDTA-treated polysomes from rabbit reticulocytes into two bands of similar intensity. Fractions containing first 10s RNA1, both 10s RNAs, and nearly-pure second 10s RNA were obtained and tested in a cell-free system derived from ascites tumor cells. These RNA preparations directed the synthesis of product composed of 85-100% α chains, 55-70% β chains, and 75-87% β chains, respectively. The relative amounts of the two 10s RNAs in certain fractions were determined by formamide electrophoresis and correlated well with the relative synthesis of β and α chains directed by these fractions in the cell-free system. Therefore, the α and β globin mRNAs are separated by this procedure. Moreover, electrophoresis of globin mRNA further purified by oligo-dT cellulose chromatography indicates that the concentration of α mRNA exceeds that of β mRNA on rabbit reticulocytes polysomes.

Original languageEnglish (US)
Pages (from-to)1053-1061
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume59
Issue number3
DOIs
StatePublished - Aug 5 1974

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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