Electrophoresis on 7.5% polyacrylamide gels in 98% formamide splits the 10s RNA of EDTA-treated polysomes from rabbit reticulocytes into two bands of similar intensity. Fractions containing first 10s RNA1, both 10s RNAs, and nearly-pure second 10s RNA were obtained and tested in a cell-free system derived from ascites tumor cells. These RNA preparations directed the synthesis of product composed of 85-100% α chains, 55-70% β chains, and 75-87% β chains, respectively. The relative amounts of the two 10s RNAs in certain fractions were determined by formamide electrophoresis and correlated well with the relative synthesis of β and α chains directed by these fractions in the cell-free system. Therefore, the α and β globin mRNAs are separated by this procedure. Moreover, electrophoresis of globin mRNA further purified by oligo-dT cellulose chromatography indicates that the concentration of α mRNA exceeds that of β mRNA on rabbit reticulocytes polysomes.
|Original language||English (US)|
|Number of pages||9|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Aug 5 1974|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology