@inbook{7b54fc157c084b6e952a6f4d4fddaeb8,
title = "Separation and Purification of multiply acetylated proteins using cation-exchange chromatography",
abstract = "High-performance liquid chromatography (HPLC) is extremely useful for the study of proteins and the characterization of their posttranslational modifications. Here we describe a method that utilizes cationexchange HPLC to separate multiply acetylated histone H3 species on the basis of their charge and hydrophilicity. This high-resolution method allows for the separation of histone H3 species that differ by as few as one acetyl group, and is compatible with subsequent analysis by a variety of techniques, including mass spectrometry and western blotting.",
keywords = "Acetic anhydride, Acetylation, Cation-exchange chromatography, HPLC, Histone H3, PolyCAT A",
author = "Romeo Papazyan and Taverna, {Sean D.}",
year = "2013",
doi = "10.1007/978-1-62703-305-3_8",
language = "English (US)",
isbn = "9781627033046",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "103--113",
booktitle = "Protein Acetylation",
}