Separation and purification of glucosinolates from crude plant homogenates by high-speed counter-current chromatography

Jed W Fahey, Kristina L. Wade, Katherine K. Stephenson, F. Edward Chou

Research output: Contribution to journalArticle

Abstract

Glucosinolates are anionic, hydrophilic plant secondary metabolites which are of particular interest due to their role in the prevention of cancer and other chronic and degenerative diseases. The separation and purification of glucosinolates from a variety of plant sources (e.g. seeds of broccoli, arugula and the horseradish tree), was achieved using high-speed counter-current chromatography (HSCCC). A high-salt, highly polar system containing 1-propanol-acetonitrile-saturated aqueous ammonium sulfate-water (1:0.5:1.2:1), was run on a semi-preparative scale and then transferred directly to preparative scale. Up to 7 g of a concentrated methanolic syrup containing about 10% glucosinolates was loaded on an 850-ml HSCCC column, and good separation and recovery were demonstrated for 4-methylsulfinylbutyl, 3-methylsulfinylpropyl, 4-methylthiobutyl, 2-propenyl and 4-(rhamnopyranosyloxy)benzyl glucosinolates. Multiple injections (5 to 6 times) were performed with well-preserved liquid stationary phase under centrifugal force. Pooled sequential runs with broccoli seed extract yielded about 20 g of its predominant glucosinolate, glucoraphanin, which was produced at >95% purity and reduced to powdered form.

Original languageEnglish (US)
Pages (from-to)85-93
Number of pages9
JournalJournal of Chromatography A
Volume996
Issue number1-2
DOIs
StatePublished - May 9 2003

Fingerprint

Glucosinolates
Chromatography
Purification
Brassica
Seed
Seeds
Armoracia
1-Propanol
Column chromatography
Ammonium Sulfate
Metabolites
Chronic Disease
Salts
Recovery
Injections
Water
Liquids
Neoplasms

Keywords

  • Brassica spp.
  • Counter-current chromatography
  • Glucoraphanin
  • Glucosinolates
  • Plant materials
  • Vegetables

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Separation and purification of glucosinolates from crude plant homogenates by high-speed counter-current chromatography. / Fahey, Jed W; Wade, Kristina L.; Stephenson, Katherine K.; Chou, F. Edward.

In: Journal of Chromatography A, Vol. 996, No. 1-2, 09.05.2003, p. 85-93.

Research output: Contribution to journalArticle

Fahey, Jed W ; Wade, Kristina L. ; Stephenson, Katherine K. ; Chou, F. Edward. / Separation and purification of glucosinolates from crude plant homogenates by high-speed counter-current chromatography. In: Journal of Chromatography A. 2003 ; Vol. 996, No. 1-2. pp. 85-93.
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