TY - JOUR
T1 - Separation and Functional Studies of the Human Lymphokine-activated Killer Cell
AU - Roberts, Kevan
AU - Lotze, Michael T.
AU - Rosenberg, Steven A.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1987/8
Y1 - 1987/8
N2 - Cell separation studies were undertaken in an attempt to purify the lymphokine-activated killer (LAK) precursor cell. Null cells, prepared by the sequential depletion of monocytes, T- and B-lymphocytes from human peripheral blood mononuclear cells, were found to be potent mediators of LAK activity. Such preparations were Leu-11+ but Leu-4- and displayed high levels of natural killer activity. Incubation of these cells with recombinant interleukin 2 (IL-2) for periods in excess of 24 h induced LAK lysis of fresh tumor targets which were resistant to lysis by unstimulated null effectors. In contrast, lymphocytes which formed high affinity rosettes with sheep RBC (E+ lymphocytes) were poor mediators of both natural killer and LAK activity. Interleukin 2 stimulated null cells, retained a Leu-11% Leu-4- phenotype, and expressed only low levels of receptors for IL-2 and transferrin. An increase in the number of binding sites, on null cells but not on T-cells, for Vicia rillosa lectin with IL-2 stimulation was noted. Following IL-2 stimulation, null and T-cells were able to conjugate to K562 and fresh tumor but not to autologous lymphoblast targets.
AB - Cell separation studies were undertaken in an attempt to purify the lymphokine-activated killer (LAK) precursor cell. Null cells, prepared by the sequential depletion of monocytes, T- and B-lymphocytes from human peripheral blood mononuclear cells, were found to be potent mediators of LAK activity. Such preparations were Leu-11+ but Leu-4- and displayed high levels of natural killer activity. Incubation of these cells with recombinant interleukin 2 (IL-2) for periods in excess of 24 h induced LAK lysis of fresh tumor targets which were resistant to lysis by unstimulated null effectors. In contrast, lymphocytes which formed high affinity rosettes with sheep RBC (E+ lymphocytes) were poor mediators of both natural killer and LAK activity. Interleukin 2 stimulated null cells, retained a Leu-11% Leu-4- phenotype, and expressed only low levels of receptors for IL-2 and transferrin. An increase in the number of binding sites, on null cells but not on T-cells, for Vicia rillosa lectin with IL-2 stimulation was noted. Following IL-2 stimulation, null and T-cells were able to conjugate to K562 and fresh tumor but not to autologous lymphoblast targets.
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M3 - Article
C2 - 3111684
AN - SCOPUS:0023278887
SN - 0008-5472
VL - 47
SP - 4366
EP - 4371
JO - Cancer Research
JF - Cancer Research
IS - 16
ER -