Abstract
Soluble arylsulfatase (EC 3.1.6.1) is present in the body fluids of man in the form of two isoenzymes, arylsulfatase A and B, which reportedly are useful biochemical markers for certain types of malignancy. However, rapid assay of the individual isoenzymes is extremely difficult; procedures based on differential inhibition or activation of the isoenzymes in a mixture yield only semiquantitative results. A feature of these isoenzymes is their inhibition by some common anions (notably phosphate) at physiologic concentrations. The isoenzymes can be separated by anion-exchange chromatography, the B isoenzyme being eluted in the void volume and the A isoenzyme and the anionic inhibitors retarded. Lead is used to sequester phosphate, enabling measurement of A in the salt-eluted fraction. Using this technique, we have found significant elevations of B in the sera of patients with colorectal cancer. The potential of rapid, chromatographic separation coupled with continuous monitoring for arylsulfatase activity is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1305-1316 |
| Number of pages | 12 |
| Journal | Clinical chemistry |
| Volume | 24 |
| Issue number | 8 |
| DOIs | |
| State | Published - 1978 |
| Externally published | Yes |
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical