Separate and variably shaped chromosome arm domains are disclosed by chromosome arm painting in human cell nuclei

Steffen Dietzel, Anna Jauch, Dirk Kienle, Guoquiong Qu, Heidi Holtgreve-Grez, Roland Eils, Christian Münkel, Michael Bittner, Paul S. Meltzer, Jeffrey M. Trent, Thomas Cremer

Research output: Contribution to journalArticle

Abstract

Fluorescence in situ hybridization (FISH) with microdissection probes from human chromosomes 3 and 6 was applied to visualize arm and subregional band domains in human amniotic fluid cell nuclei. Confocal laser scanning microscopy and quantitative three-dimensional image analysis showed a pronounced variability of p- and q-arm domain arrangements and shapes. Apparent intermingling of neighbouring arm domains was limited to the domain surface. Three-dimensional distance measurements with pter and qter probes supported a high variability of chromosome territory folding.

Original languageEnglish (US)
Pages (from-to)25-33
Number of pages9
JournalChromosome Research
Volume6
Issue number1
DOIs
StatePublished - Feb 18 1998

Keywords

  • Chromosome arms
  • Chromosome structure
  • Chromosome territories
  • Confocal microscopy
  • Fluorescence in situ hybridization
  • Nuclear organization
  • Random walk/giant loop model

ASJC Scopus subject areas

  • Genetics

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    Dietzel, S., Jauch, A., Kienle, D., Qu, G., Holtgreve-Grez, H., Eils, R., Münkel, C., Bittner, M., Meltzer, P. S., Trent, J. M., & Cremer, T. (1998). Separate and variably shaped chromosome arm domains are disclosed by chromosome arm painting in human cell nuclei. Chromosome Research, 6(1), 25-33. https://doi.org/10.1023/A:1009262223693