Sensitivity of sandwich enzyme-linked immunosorbent assay for cryptococcus neoformans polysaccharide antigen is dependent on the isotypes of the capture and detection antibodies

S. Mukherjee, A. Casadevall

Research output: Contribution to journalComment/debate

Abstract

Immunoglobulin M (IgM) and IgG1 monoclonal antibody isotype switch variants to Cryptococcus neoformans capsular polysaccharide were used to study the sensitivity of a double sandwich enzyme-linked immunosorbent assay (ELISA). The most sensitive ELISA configurations used IgG1 monoclonal antibody absorbed on polystyrene plates or IgM immobilized with goat antisera for antigen capture.

Original languageEnglish (US)
Pages (from-to)765-768
Number of pages4
JournalJournal of clinical microbiology
Volume33
Issue number3
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Microbiology (medical)

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