Sensitivity of sandwich enzyme-linked immunosorbent assay for cryptococcus neoformans polysaccharide antigen is dependent on the isotypes of the capture and detection antibodies

S. Mukherjee; A. Casadevall

doi:10.1128/jcm.33.3.765-768.1995

Sensitivity of sandwich enzyme-linked immunosorbent assay for cryptococcus neoformans polysaccharide antigen is dependent on the isotypes of the capture and detection antibodies

S. Mukherjee, A. Casadevall

Research output: Contribution to journal › Comment/debate › peer-review

7 Scopus citations

Abstract

Immunoglobulin M (IgM) and IgG1 monoclonal antibody isotype switch variants to Cryptococcus neoformans capsular polysaccharide were used to study the sensitivity of a double sandwich enzyme-linked immunosorbent assay (ELISA). The most sensitive ELISA configurations used IgG1 monoclonal antibody absorbed on polystyrene plates or IgM immobilized with goat antisera for antigen capture.

Original language	English (US)
Pages (from-to)	765-768
Number of pages	4
Journal	Journal of clinical microbiology
Volume	33
Issue number	3
DOIs	https://doi.org/10.1128/jcm.33.3.765-768.1995
State	Published - 1995
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/jcm.33.3.765-768.1995

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title = "Sensitivity of sandwich enzyme-linked immunosorbent assay for cryptococcus neoformans polysaccharide antigen is dependent on the isotypes of the capture and detection antibodies",

abstract = "Immunoglobulin M (IgM) and IgG1 monoclonal antibody isotype switch variants to Cryptococcus neoformans capsular polysaccharide were used to study the sensitivity of a double sandwich enzyme-linked immunosorbent assay (ELISA). The most sensitive ELISA configurations used IgG1 monoclonal antibody absorbed on polystyrene plates or IgM immobilized with goat antisera for antigen capture.",

author = "S. Mukherjee and A. Casadevall",

year = "1995",

doi = "10.1128/jcm.33.3.765-768.1995",

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AU - Casadevall, A.

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AB - Immunoglobulin M (IgM) and IgG1 monoclonal antibody isotype switch variants to Cryptococcus neoformans capsular polysaccharide were used to study the sensitivity of a double sandwich enzyme-linked immunosorbent assay (ELISA). The most sensitive ELISA configurations used IgG1 monoclonal antibody absorbed on polystyrene plates or IgM immobilized with goat antisera for antigen capture.

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Sensitivity of sandwich enzyme-linked immunosorbent assay for cryptococcus neoformans polysaccharide antigen is dependent on the isotypes of the capture and detection antibodies

Abstract

ASJC Scopus subject areas

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