Sensitivity enhancement in NMR of macromolecules by application of optimal control theory

Dominique P. Frueh, Takuhiro Ito, Jr Shin Li, Gerhard Wagner, Steffen J. Glaser, Navin Khaneja

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


NMR of macromolecules is limited by large transverse relaxation rates. In practice, this results in low efficiency of coherence transfer steps in multidimensional NMR experiments, leading to poor sensitivity and long acquisition times. The efficiency of coherence transfer can be maximized by design of relaxation optimized pulse sequences using tools from optimal control theory. In this paper, we demonstrate that this approach can be adopted for studies of large biological systems, such as the 800 kDa chaperone GroEL. For this system, the 1H- 15N coherence transfer module presented here yields an average sensitivity enhancement of 20-25% for cross-correlated relaxation induced polarization transfer (CRIPT) experiments.

Original languageEnglish (US)
Pages (from-to)23-30
Number of pages8
JournalJournal of Biomolecular NMR
Issue number1
StatePublished - May 2005
Externally publishedYes


  • Cross-correlation
  • GroEL
  • Macromolecular NMR
  • Optimal control
  • Relaxation

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy


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