Sensitive drug-resistance assays reveal long-term persistence of HIV-1 variants with the K103N nevirapine (NVP) resistance mutation in some women and infants after the administration of single-dose NVP: HIVNET 012

Tamara Flys, Dwight V. Nissley, Cassidy W. Claasen, Dana Jones, Chanjuan Shi, Laura A. Guay, Philippa Musoke, Francis Mmiro, Jeffrey N. Strathern, J. Brooks Jackson, James R. Eshleman, Susan H. Eshleman

Research output: Contribution to journalArticle

Abstract

Background. The HIV Network for Prevention Trials (HIVNET) 012 trial showed that NVP resistance (NVPR) emerged in some women and children after the administration of single-dose nevirapine (SD-NVP). We tested whether K103N-containing human immunodeficiency virus (HIV)-1 variants persisted in women and infants 1 year or more after the administration of SD-NVP. Methods. We analyzed samples from 9 women and 5 infants in HIVNET 012 who had NVPR 6-8 weeks after the administration of SD-NVP. Samples were analyzed with the ViroSeq system and with 2 sensitive resistance assays, LigAmp and TyHRT. Results. ViroSeq detected the K103N mutation in 8 of 9 women and in 2 of 5 infants. LigAmp detected the K103N mutation at low levels in 8 of 9 women and in 4 of 5 infants. K103N was not detected by ViroSeq 12-24 months after the administration of SD-NVP but was detected by LigAmp in 3 of 9 women and in 1 of 5 infants. K103N was also detected in those samples by use of the TyHRT assay. Conclusions. K103N-containing variants persist in some women and infants for 1 year or more after the administration of SD-NVP. Sensitive resistance assays may provide new insight into the impact of antiretroviral drug exposure on HIV-1 evolution.

Original languageEnglish (US)
Pages (from-to)24-29
Number of pages6
JournalJournal of Infectious Diseases
Volume192
Issue number1
DOIs
StatePublished - Jul 1 2005

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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