Sensitive and rapid method for amino acid quantitation in malaria biological samples using AccQ • Tag ultra performance liquid chromatography-electrospray ionization-MS/MS with multiple reaction monitoring

Jenny M. Armenta, Diego F. Cortes, John M. Pisciotta, Joel L. Shuman, Kenneth Blakeslee, Dominique Rasoloson, Oluwatosin Ogunbiyi, David J Sullivan, Vladimir Shulaev

Research output: Contribution to journalArticle

Abstract

An AccQ • Tag ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (AccQ•Tag-UPLC-ESI-MS/MS) method for fast, reproducible, and sensitive amino acid quantitation in biological samples, particularly, the malaria parasite Plasmodium falciparum is presented. The Waters Acquity TQD UPLC/MS system equipped with a photodiode array (PDA) detector was used for amino acid separation and detection. The method was developed and validated using amino acid standard mixtures containing acidic, neutral, and basic amino acids. For MS analysis, the optimum cone voltage implemented, based on direct infusion analysis of a few selected AccQ•Tag amino acids with multiple reaction monitoring, varied from 29 to 39 V, whereas the collision energy varied from 15 to 35 V. Calibration curves were built using both internal and external standardization. Typically, a linear response for all amino acids was observed at concentration ranges of 3 × 10 -3-25 pmol/μL. For some amino acids, concentration limits of detection were as low as 1.65 fmol. The coefficients of variation for retention times were within the range of 0.08 - 1.08%. The coefficients of variation for amino acid quantitation, determined from triplicate UPLC-MS/MS runs, were below 8% on the average. The developed AccQ•Tag-UPLC-ESI-MS/MS method revealed good technical and biological reproducibility when applied to P. falciparum and human red blood cells samples. This study should provide a valuable insight into the performance of UPLC-ESI-MS/MS for amino acid quantitation using AccQ•Tag derivatization.

Original languageEnglish (US)
Pages (from-to)548-558
Number of pages11
JournalAnalytical Chemistry
Volume82
Issue number2
DOIs
StatePublished - Jan 15 2010

Fingerprint

Electrospray ionization
Liquid chromatography
Amino Acids
Monitoring
Acidic Amino Acids
Neutral Amino Acids
Basic Amino Acids
Photodiodes
Standardization
Mass spectrometry
Cones
Blood
Cells
Calibration
Detectors
Water
Electric potential

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Sensitive and rapid method for amino acid quantitation in malaria biological samples using AccQ • Tag ultra performance liquid chromatography-electrospray ionization-MS/MS with multiple reaction monitoring. / Armenta, Jenny M.; Cortes, Diego F.; Pisciotta, John M.; Shuman, Joel L.; Blakeslee, Kenneth; Rasoloson, Dominique; Ogunbiyi, Oluwatosin; Sullivan, David J; Shulaev, Vladimir.

In: Analytical Chemistry, Vol. 82, No. 2, 15.01.2010, p. 548-558.

Research output: Contribution to journalArticle

Armenta, Jenny M. ; Cortes, Diego F. ; Pisciotta, John M. ; Shuman, Joel L. ; Blakeslee, Kenneth ; Rasoloson, Dominique ; Ogunbiyi, Oluwatosin ; Sullivan, David J ; Shulaev, Vladimir. / Sensitive and rapid method for amino acid quantitation in malaria biological samples using AccQ • Tag ultra performance liquid chromatography-electrospray ionization-MS/MS with multiple reaction monitoring. In: Analytical Chemistry. 2010 ; Vol. 82, No. 2. pp. 548-558.
@article{078946f277e349caa0c24f9fed13a611,
title = "Sensitive and rapid method for amino acid quantitation in malaria biological samples using AccQ • Tag ultra performance liquid chromatography-electrospray ionization-MS/MS with multiple reaction monitoring",
abstract = "An AccQ • Tag ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (AccQ•Tag-UPLC-ESI-MS/MS) method for fast, reproducible, and sensitive amino acid quantitation in biological samples, particularly, the malaria parasite Plasmodium falciparum is presented. The Waters Acquity TQD UPLC/MS system equipped with a photodiode array (PDA) detector was used for amino acid separation and detection. The method was developed and validated using amino acid standard mixtures containing acidic, neutral, and basic amino acids. For MS analysis, the optimum cone voltage implemented, based on direct infusion analysis of a few selected AccQ•Tag amino acids with multiple reaction monitoring, varied from 29 to 39 V, whereas the collision energy varied from 15 to 35 V. Calibration curves were built using both internal and external standardization. Typically, a linear response for all amino acids was observed at concentration ranges of 3 × 10 -3-25 pmol/μL. For some amino acids, concentration limits of detection were as low as 1.65 fmol. The coefficients of variation for retention times were within the range of 0.08 - 1.08{\%}. The coefficients of variation for amino acid quantitation, determined from triplicate UPLC-MS/MS runs, were below 8{\%} on the average. The developed AccQ•Tag-UPLC-ESI-MS/MS method revealed good technical and biological reproducibility when applied to P. falciparum and human red blood cells samples. This study should provide a valuable insight into the performance of UPLC-ESI-MS/MS for amino acid quantitation using AccQ•Tag derivatization.",
author = "Armenta, {Jenny M.} and Cortes, {Diego F.} and Pisciotta, {John M.} and Shuman, {Joel L.} and Kenneth Blakeslee and Dominique Rasoloson and Oluwatosin Ogunbiyi and Sullivan, {David J} and Vladimir Shulaev",
year = "2010",
month = "1",
day = "15",
doi = "10.1021/ac901790q",
language = "English (US)",
volume = "82",
pages = "548--558",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "2",

}

TY - JOUR

T1 - Sensitive and rapid method for amino acid quantitation in malaria biological samples using AccQ • Tag ultra performance liquid chromatography-electrospray ionization-MS/MS with multiple reaction monitoring

AU - Armenta, Jenny M.

AU - Cortes, Diego F.

AU - Pisciotta, John M.

AU - Shuman, Joel L.

AU - Blakeslee, Kenneth

AU - Rasoloson, Dominique

AU - Ogunbiyi, Oluwatosin

AU - Sullivan, David J

AU - Shulaev, Vladimir

PY - 2010/1/15

Y1 - 2010/1/15

N2 - An AccQ • Tag ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (AccQ•Tag-UPLC-ESI-MS/MS) method for fast, reproducible, and sensitive amino acid quantitation in biological samples, particularly, the malaria parasite Plasmodium falciparum is presented. The Waters Acquity TQD UPLC/MS system equipped with a photodiode array (PDA) detector was used for amino acid separation and detection. The method was developed and validated using amino acid standard mixtures containing acidic, neutral, and basic amino acids. For MS analysis, the optimum cone voltage implemented, based on direct infusion analysis of a few selected AccQ•Tag amino acids with multiple reaction monitoring, varied from 29 to 39 V, whereas the collision energy varied from 15 to 35 V. Calibration curves were built using both internal and external standardization. Typically, a linear response for all amino acids was observed at concentration ranges of 3 × 10 -3-25 pmol/μL. For some amino acids, concentration limits of detection were as low as 1.65 fmol. The coefficients of variation for retention times were within the range of 0.08 - 1.08%. The coefficients of variation for amino acid quantitation, determined from triplicate UPLC-MS/MS runs, were below 8% on the average. The developed AccQ•Tag-UPLC-ESI-MS/MS method revealed good technical and biological reproducibility when applied to P. falciparum and human red blood cells samples. This study should provide a valuable insight into the performance of UPLC-ESI-MS/MS for amino acid quantitation using AccQ•Tag derivatization.

AB - An AccQ • Tag ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (AccQ•Tag-UPLC-ESI-MS/MS) method for fast, reproducible, and sensitive amino acid quantitation in biological samples, particularly, the malaria parasite Plasmodium falciparum is presented. The Waters Acquity TQD UPLC/MS system equipped with a photodiode array (PDA) detector was used for amino acid separation and detection. The method was developed and validated using amino acid standard mixtures containing acidic, neutral, and basic amino acids. For MS analysis, the optimum cone voltage implemented, based on direct infusion analysis of a few selected AccQ•Tag amino acids with multiple reaction monitoring, varied from 29 to 39 V, whereas the collision energy varied from 15 to 35 V. Calibration curves were built using both internal and external standardization. Typically, a linear response for all amino acids was observed at concentration ranges of 3 × 10 -3-25 pmol/μL. For some amino acids, concentration limits of detection were as low as 1.65 fmol. The coefficients of variation for retention times were within the range of 0.08 - 1.08%. The coefficients of variation for amino acid quantitation, determined from triplicate UPLC-MS/MS runs, were below 8% on the average. The developed AccQ•Tag-UPLC-ESI-MS/MS method revealed good technical and biological reproducibility when applied to P. falciparum and human red blood cells samples. This study should provide a valuable insight into the performance of UPLC-ESI-MS/MS for amino acid quantitation using AccQ•Tag derivatization.

UR - http://www.scopus.com/inward/record.url?scp=75649092232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=75649092232&partnerID=8YFLogxK

U2 - 10.1021/ac901790q

DO - 10.1021/ac901790q

M3 - Article

C2 - 20038084

AN - SCOPUS:75649092232

VL - 82

SP - 548

EP - 558

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 2

ER -