Semi-automated optical Heartbeat Analysis of small hearts

Karen Ocorr, Martin Fink, Anthony Ross Cammarato, Sanford I. Bernstein, Rolf Bodmer

Research output: Contribution to journalArticle

Abstract

We have developed a method for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts (Fink, et. al., 2009). Our Semi-automatic Optical Heartbeat Analysis (SOHA) uses a novel movement detection algorithm that is able to detect cardiac movements associated with individual contractile and relaxation events. The program provides a host of physiologically relevant readouts including systolic and diastolic intervals, heart rate, as well as qualitative and quantitative measures of heartbeat arrhythmicity. The program also calculates heart diameter measurements during both diastole and systole from which fractional shortening and fractional area changes are calculated. Output is provided as a digital file compatible with most spreadsheet programs. Measurements are made for every heartbeat in a record increasing the statistical power of the output. We demonstrate each of the steps where user input is required and show the application of our methodology to the analysis of heart function in all three genetically tractable heart models.

Original languageEnglish (US)
Article numbere1435
JournalJournal of Visualized Experiments
Issue number31
DOIs
StatePublished - Sep 2009
Externally publishedYes

Fingerprint

Diastole
Systole
Zebrafish
Optical recording
Drosophila
Spreadsheets
Heart Rate

Keywords

  • Cardiomyopathy
  • Dilated
  • Drosophila
  • Heart
  • Issue 31
  • KCNQ
  • Mouse
  • Movement detection
  • Myosin
  • Physiology
  • Restricted
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Semi-automated optical Heartbeat Analysis of small hearts. / Ocorr, Karen; Fink, Martin; Cammarato, Anthony Ross; Bernstein, Sanford I.; Bodmer, Rolf.

In: Journal of Visualized Experiments, No. 31, e1435, 09.2009.

Research output: Contribution to journalArticle

Ocorr, Karen ; Fink, Martin ; Cammarato, Anthony Ross ; Bernstein, Sanford I. ; Bodmer, Rolf. / Semi-automated optical Heartbeat Analysis of small hearts. In: Journal of Visualized Experiments. 2009 ; No. 31.
@article{e767c39bacd1424aa2348bc986e50795,
title = "Semi-automated optical Heartbeat Analysis of small hearts",
abstract = "We have developed a method for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts (Fink, et. al., 2009). Our Semi-automatic Optical Heartbeat Analysis (SOHA) uses a novel movement detection algorithm that is able to detect cardiac movements associated with individual contractile and relaxation events. The program provides a host of physiologically relevant readouts including systolic and diastolic intervals, heart rate, as well as qualitative and quantitative measures of heartbeat arrhythmicity. The program also calculates heart diameter measurements during both diastole and systole from which fractional shortening and fractional area changes are calculated. Output is provided as a digital file compatible with most spreadsheet programs. Measurements are made for every heartbeat in a record increasing the statistical power of the output. We demonstrate each of the steps where user input is required and show the application of our methodology to the analysis of heart function in all three genetically tractable heart models.",
keywords = "Cardiomyopathy, Dilated, Drosophila, Heart, Issue 31, KCNQ, Mouse, Movement detection, Myosin, Physiology, Restricted, Zebrafish",
author = "Karen Ocorr and Martin Fink and Cammarato, {Anthony Ross} and Bernstein, {Sanford I.} and Rolf Bodmer",
year = "2009",
month = "9",
doi = "10.3791/1435",
language = "English (US)",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "31",

}

TY - JOUR

T1 - Semi-automated optical Heartbeat Analysis of small hearts

AU - Ocorr, Karen

AU - Fink, Martin

AU - Cammarato, Anthony Ross

AU - Bernstein, Sanford I.

AU - Bodmer, Rolf

PY - 2009/9

Y1 - 2009/9

N2 - We have developed a method for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts (Fink, et. al., 2009). Our Semi-automatic Optical Heartbeat Analysis (SOHA) uses a novel movement detection algorithm that is able to detect cardiac movements associated with individual contractile and relaxation events. The program provides a host of physiologically relevant readouts including systolic and diastolic intervals, heart rate, as well as qualitative and quantitative measures of heartbeat arrhythmicity. The program also calculates heart diameter measurements during both diastole and systole from which fractional shortening and fractional area changes are calculated. Output is provided as a digital file compatible with most spreadsheet programs. Measurements are made for every heartbeat in a record increasing the statistical power of the output. We demonstrate each of the steps where user input is required and show the application of our methodology to the analysis of heart function in all three genetically tractable heart models.

AB - We have developed a method for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts (Fink, et. al., 2009). Our Semi-automatic Optical Heartbeat Analysis (SOHA) uses a novel movement detection algorithm that is able to detect cardiac movements associated with individual contractile and relaxation events. The program provides a host of physiologically relevant readouts including systolic and diastolic intervals, heart rate, as well as qualitative and quantitative measures of heartbeat arrhythmicity. The program also calculates heart diameter measurements during both diastole and systole from which fractional shortening and fractional area changes are calculated. Output is provided as a digital file compatible with most spreadsheet programs. Measurements are made for every heartbeat in a record increasing the statistical power of the output. We demonstrate each of the steps where user input is required and show the application of our methodology to the analysis of heart function in all three genetically tractable heart models.

KW - Cardiomyopathy

KW - Dilated

KW - Drosophila

KW - Heart

KW - Issue 31

KW - KCNQ

KW - Mouse

KW - Movement detection

KW - Myosin

KW - Physiology

KW - Restricted

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=80055117330&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80055117330&partnerID=8YFLogxK

U2 - 10.3791/1435

DO - 10.3791/1435

M3 - Article

C2 - 19759521

AN - SCOPUS:80055117330

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 31

M1 - e1435

ER -