Semaphorin 3A contributes to distal pulmonary epithelial cell differentiation and lung morphogenesis

Patrice M. Becker, Tracy S. Tran, Michael J. Delannoy, Chaoxia He, John M. Shannon, Sharon A McGrath-Morrow

Research output: Contribution to journalArticle

Abstract

Rationale: Semaphorin 3A (Sema3A) is a neural guidance cue that also mediates cell migration, proliferation and apoptosis, and inhibits branching morphogenesis. Because we have shown that genetic deletion of neuropilin-1, which encodes an obligatory Sema3A co-receptor, influences airspace remodeling in the smoke-exposed adult lung, we sought to determine whether genetic deletion of Sema3A altered distal lung structure. Methods: To determine whether loss of Sema3A signaling influenced distal lung morphology, we compared pulmonary histology, distal epithelial cell morphology and maturation, and the balance between lung cell proliferation and death, in lungs from mice with a targeted genetic deletion of Sema3A (Sema3A -/-) and wild-type (Sema3A +/+) littermate controls. Results: Genetic deletion of Sema3A resulted in significant perinatal lethality. At E17.5, lungs from Sema3A -/- mice had thickened septae and reduced airspace size. Distal lung epithelial cells had increased intracellular glycogen pools and small multivesicular and lamellar bodies with atypical ultrastructure, as well as reduced expression of type I alveolar epithelial cell markers. Alveolarization was markedly attenuated in lungs from the rare Sema3A -/- mice that survived the immediate perinatal period. Furthermore, Sema3A deletion was linked with enhanced postnatal alveolar septal cell death. Conclusions: These data suggest that Sema3A modulates distal pulmonary epithelial cell development and alveolar septation. Defining how Sema3A influences structural plasticity of the developing lung is a critical first step for determining if this pathway can be exploited to develop innovative strategies for repair after acute or chronic lung injury.

Original languageEnglish (US)
Article numbere27449
JournalPLoS One
Volume6
Issue number11
DOIs
StatePublished - Nov 11 2011

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Semaphorin-3A
Morphogenesis
cell differentiation
morphogenesis
Cell Differentiation
epithelial cells
Epithelial Cells
lungs
Lung
Alveolar Epithelial Cells
Cell death
cell death
mice
Cell Death
Neuropilin-1
Cell Proliferation
keratinosomes
perinatal period
Multivesicular Bodies
Histology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Semaphorin 3A contributes to distal pulmonary epithelial cell differentiation and lung morphogenesis. / Becker, Patrice M.; Tran, Tracy S.; Delannoy, Michael J.; He, Chaoxia; Shannon, John M.; McGrath-Morrow, Sharon A.

In: PLoS One, Vol. 6, No. 11, e27449, 11.11.2011.

Research output: Contribution to journalArticle

Becker, Patrice M. ; Tran, Tracy S. ; Delannoy, Michael J. ; He, Chaoxia ; Shannon, John M. ; McGrath-Morrow, Sharon A. / Semaphorin 3A contributes to distal pulmonary epithelial cell differentiation and lung morphogenesis. In: PLoS One. 2011 ; Vol. 6, No. 11.
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